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      Directed differentiation of telencephalic precursors from embryonic stem cells.

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          Abstract

          We demonstrate directed differentiation of telencephalic precursors from mouse embryonic stem (ES) cells using optimized serum-free suspension culture (SFEB culture). Treatment with Wnt and Nodal antagonists (Dkk1 and LeftyA) during the first 5 d of SFEB culture causes nearly selective neural differentiation in ES cells ( approximately 90%). In the presence of Dkk1, with or without LeftyA, SFEB induces efficient generation ( approximately 35%) of cells expressing telencephalic marker Bf1. Wnt3a treatment during the late culture period increases the pallial telencephalic population (Pax6(+) cells yield up to 75% of Bf1(+) cells), whereas Shh promotes basal telencephalic differentiation (into Nkx2.1(+) and/or Islet1/2(+) cells) at the cost of pallial telencephalic differentiation. Thus, in the absence of caudalizing signals, floating aggregates of ES cells generate naive telencephalic precursors that acquire subregional identities by responding to extracellular patterning signals.

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          Author and article information

          Journal
          Nat Neurosci
          Nature neuroscience
          Springer Science and Business Media LLC
          1097-6256
          1097-6256
          Mar 2005
          : 8
          : 3
          Affiliations
          [1 ] Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan.
          Article
          nn1402
          10.1038/nn1402
          15696161
          400c7132-b0d4-4674-a79d-a7ce1f8a43ad
          History

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