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      Characterization of mucilage polysaccharides, arabinogalactanproteins and cell-wall hemicellulosic polysaccharides isolated from flax seed meal: A wealth of structural moieties.

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          Abstract

          The present study aimed at analyzing the structural features of seed mucilage and cell-wall polysaccharides which accounted for 41% of the mass of flax meal (FM). A combination of high molar-mass mucilage-like polysaccharides (rhamnogalacturonan and arabinoxylan) was released from FM in water, together with arabinogalactan proteins and glucans. About half of FM homogalacturonans was extracted using a calcium chelator and boiling water. Hemicellulosic xyloglucans and xylans were further extracted with 1M KOH, in ∼13% FM-sugars yield. Structural characterization of the xyloglucan using specific enzyme hydrolysis, ion exchange chromatography (HPAEC) and matrix assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectroscopy showed the presence of XXXG type xyloglucan, but also that of XXGG-structure, possibly characteristic of flax seeds. Hydrolysis of xylans with endo-(1→4)-β-D-xylanase, and analysis of the neutral and acidic oligosaccharides by MALDI-TOF-MS showed that xylan consisted of β-(1→4)-linked-D-xylopyranose backbone with some zones (DP 5-7) substituted with 4-O-MeGlcA\GlcA\Glc residues.

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          Author and article information

          Journal
          Carbohydr Polym
          Carbohydrate polymers
          Elsevier BV
          1879-1344
          0144-8617
          Apr 02 2013
          : 93
          : 2
          Affiliations
          [1 ] Natural Products Laboratory, Department of Chemistry, The University of Burdwan, WB 713 104, India.
          Article
          S0144-8617(12)01257-X
          10.1016/j.carbpol.2012.12.034
          23499108
          40226ffc-b14f-4959-b575-d53a735880f8
          Copyright © 2012 Elsevier Ltd. All rights reserved.
          History

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