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      Immunoreactive Delta Sleep-Inducing Peptide Secretion from Mouse Dissociated, Anterior Pituitary Cells: Regulation by Corticotropin-Releasing Factor and Arginine Vasopressin

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          Immunoreactive delta sleep-inducing peptide (IR-DSIP) has previously been localized to the ACTH/MSH cells of the human and porcine pituitary gland. In the present report, the distribution of IR-DSIP in the mouse pituitary gland was examined by immunocytochemistry. In this species, IR-DSIP was found to be co-localized with thyroid-stimulating hormone (TSH) in anterior pituitary thyrotrophs and was also present in nerve fibers in the posterior and intermediate lobes. The effect of synthetic DSIP on IR-ACTH release from dissociated mouse anterior pituitary cells was also studied. DSIP (>10<sup>–9</sup> M) inhibited both basal and CRF-induced IR-ACTH release from these cells. In addition, the effect of synthetic rat corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) on IR-DSIP secretion was investigated. CRF and AVP at concentrations of 10<sup>–11–</sup>10<sup>–7</sup> M inhibited release of IR-DSIP from mouse anterior pituitary cells by 63%. When CRF and AVP (10<sup>–10</sup>–10<sup>–7</sup> M) were given concomitantly, the maximal inhibition of IR-DSIP release was observed at a concentration of 10<sup>–8</sup> M of CRF and AVP. However, these two peptides when given together showed no additive effect on IR-DSIP secretion. These findings suggest that during CRF induction of ACTH secretion from anterior pituitary corticotrophs, CRF may also act simultaneously to inhibit DSIP secretion from the thyrotrophs.

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          Author and article information

          S. Karger AG
          02 April 2008
          : 50
          : 5
          : 564-569
          Departments of aPsychiatry and Neurochemistry, and bMedical Cell Research, University of Lund, Sweden; cLaboratory of Neurochemistry and Neuroimmunology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Md., USA
          125282 Neuroendocrinology 1989;50:564–569
          © 1989 S. Karger AG, Basel

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          Pages: 6
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