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      Co-ordinated expression of lymphoid and myeloid specific transcription factors during B-1b cell differentiation into mononuclear phagocytes in vitro.

      Immunology
      Animals, B-Lymphocyte Subsets, immunology, metabolism, B-Lymphocytes, Cell Differentiation, Cells, Cultured, Gene Expression Profiling, methods, Gene Expression Regulation, Immunoglobulin M, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Myeloid Cells, Phagocytes, Phagocytosis, Reverse Transcriptase Polymerase Chain Reaction, Spleen, Transcription Factors

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          Abstract

          We previously demonstrated that B-1b cells can undergo differentiation to acquire a mononuclear phagocyte phenotype upon attachment to substrate in vitro. Here we followed the expression of surface markers and transcription factors during this differentiation. B-1b cells spontaneously express both myeloid and lymphoid restricted transcription factors. When induced to differentiate into a phagocyte, the lymphoid genes E box protein (E2A), early B-cell factor (EBF), paired box 5 (Pax5) are down-modulated, while expression of genes related to myeloid commitment is sustained. Furthermore, B-1b cell-derived phagocytes (B-1CDPs) decrease immunoglobulin M (IgM) expression but retain the expression of the heavy chain variable gene VH11 or VH12, an immunoglobulin gene rearrangement predominantly expressed by B-1 cells. The maintenance of lymphoid characteristics in B-1CDPs characterizes a unique type of phagocyte, not related to monocyte-derived macrophages.

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