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      Full-length, Oligomeric Structure of Wzz Determined by Cryoelectron Microscopy Reveals Insights into Membrane-Bound States.

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          Abstract

          Wzz is an integral inner membrane protein involved in regulating the length of lipopolysaccharide O-antigen glycans and essential for the virulence of many Gram-negative pathogens. In all Wzz homologs, the large periplasmic domain is proposed to be anchored by two transmembrane helices, but no information is available for the transmembrane and cytosolic domains. Here we have studied purified oligomeric Wzz complexes using cryoelectron microscopy and resolved the transmembrane regions within a semi-continuous detergent micelle. The transmembrane helices of each monomer display a right-handed super-helical twist, and do not interact with the neighboring transmembrane domains. Modeling, flexible fitting and multiscale simulation approaches were used to study the full-length complex and to provide explanations for the influence of the lipid bilayer on its oligomeric status. Based on structural and in silico observations, we propose a new mechanism for O-antigen chain-length regulation that invokes synergy of Wzz and its polymerase partner, Wzy.

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          Author and article information

          Journal
          Structure
          Structure (London, England : 1993)
          Elsevier BV
          1878-4186
          0969-2126
          May 02 2017
          : 25
          : 5
          Affiliations
          [1 ] Faculty of Biology, Medicine and Health, The University of Manchester, Dover Street, Manchester M13 9PT, UK.
          [2 ] Faculty of Biology, Medicine and Health, The University of Manchester, Dover Street, Manchester M13 9PT, UK; Bioinformatics Institute, 30 Biopolis Street, Singapore 138671, Singapore.
          [3 ] Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.
          [4 ] eBIC, Diamond Light Source Ltd, Diamond House, Harwell Science & Innovation Campus, Didcot, Oxfordshire OX11 0DE, UK.
          [5 ] Bioinformatics Institute, 30 Biopolis Street, Singapore 138671, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
          [6 ] Faculty of Biology, Medicine and Health, The University of Manchester, Dover Street, Manchester M13 9PT, UK. Electronic address: robert.ford@manchester.ac.uk.
          Article
          S0969-2126(17)30077-1
          10.1016/j.str.2017.03.017
          28434914
          40e3dde5-81ff-402a-825b-046f153a1cf9
          History

          Wzz,cryoelectron microscopy,lipopolysaccharide,molecular dynamics,multiscale simulation,transmembrane domains

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