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      Illegitimate transcription: transcription of any gene in any cell type.

      Proceedings of the National Academy of Sciences of the United States of America
      Animals, Anti-Mullerian Hormone, Factor VIII, genetics, Fibroblasts, physiology, Fructose-Bisphosphate Aldolase, Gene Amplification, Gene Expression Regulation, Globins, Glycoproteins, Growth Inhibitors, Humans, Liver Neoplasms, Experimental, Lymphocytes, Pyruvate Kinase, RNA Splicing, RNA, Messenger, Rats, Testicular Hormones, Transcription, Genetic

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          Abstract

          Using in vitro amplification of cDNA by the polymerase chain reaction, we have detected spliced transcripts of various tissue-specific genes (genes for anti-Müllerian hormone, beta-globin, aldolase A, and factor VIIIc) in human nonspecific cells, such as fibroblasts, hepatoma cells, and lymphoblasts. In rats, erythroid- and liver-type pyruvate kinase transcripts were also detected in brain, lung, and muscle. The abundance of these "illegitimate" transcripts is very low; yet, their existence and the possibility of amplifying them by the cDNA polymerase chain reaction provide a powerful tool to analyze pathological transcripts of any tissue-specific gene by using any accessible cell.

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