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      Draft Genome Sequence of Vibrio parahaemolyticus Strain PH1339, Which Causes Acute Hepatopancreatic Necrosis Disease in Shrimp in the Philippines

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          Abstract

          We report the first draft genome sequence of an acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strain isolated from a Penaeus vannamei sample from the Philippines. The strain carries the genes encoding the Pir-like toxin pair PirA vp and PirB vp.

          ABSTRACT

          We report the first draft genome sequence of an acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strain isolated from a Penaeus vannamei sample from the Philippines. The strain carries the genes encoding the Pir-like toxin pair PirA vp and PirB vp.

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          Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp.

          A new emerging disease in shrimp, first reported in 2009, was initially named early mortality syndrome (EMS). In 2011, a more descriptive name for the acute phase of the disease was proposed as acute hepatopancreatic necrosis syndrome (AHPNS). Affecting both Pacific white shrimp Penaeus vannamei and black tiger shrimp P. monodon, the disease has caused significant losses in Southeast Asian shrimp farms. AHPNS was first classified as idiopathic because no specific causative agent had been identified. However, in early 2013, the Aquaculture Pathology Laboratory at the University of Arizona was able to isolate the causative agent of AHPNS in pure culture. Immersion challenge tests were employed for infectivity studies, which induced 100% mortality with typical AHPNS pathology to experimental shrimp exposed to the pathogenic agent. Subsequent histological analyses showed that AHPNS lesions were experimentally induced in the laboratory and were identical to those found in AHPNS-infected shrimp samples collected from the endemic areas. Bacterial isolation from the experimentally infected shrimp enabled recovery of the same bacterial colony type found in field samples. In 3 separate immersion tests, using the recovered isolate from the AHPNS-positive shrimp, the same AHPNS pathology was reproduced in experimental shrimp with consistent results. Hence, AHPNS has a bacterial etiology and Koch's Postulates have been satisfied in laboratory challenge studies with the isolate, which has been identified as a member of the Vibrio harveyi clade, most closely related to V. parahemolyticus.
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            Assembling Genomes and Mini-metagenomes from Highly Chimeric Reads

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                Author and article information

                Contributors
                Role: Editor
                Journal
                Microbiol Resour Announc
                Microbiol Resour Announc
                ga
                mra
                MRA
                Microbiology Resource Announcements
                American Society for Microbiology (1752 N St., N.W., Washington, DC )
                2576-098X
                14 November 2019
                November 2019
                : 8
                : 46
                : e01020-19
                Affiliations
                [a ]National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon City, Philippines
                [b ]Philippine Genome Center, University of the Philippines, Diliman, Quezon City, Philippines
                [c ]Southeast Asian Fisheries Development Center, Aquaculture Department, Tigbauan, Iloilo, Philippines
                Georgia Institute of Technology
                Author notes
                Address correspondence to Edgar C. Amar, eamar@ 123456seafdec.org.ph , or Cynthia P. Saloma, cpsaloma@ 123456mbb.upd.edu.ph .
                [*]

                Present address: Sarah Mae U. Penir, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany; Nikko Alvin R. Cabillon, The Hebrew University of Jerusalem, Jerusalem, Israel; Angela Denise P. Bilbao, Hain Celestial Group, New Hyde Park, New York, USA.

                Citation Penir SMU, dela Pena LD, Cabillon NAR, Bilbao ADP, Amar EC, Saloma CP. 2019. Draft genome sequence of Vibrio parahaemolyticus strain PH1339, which causes acute hepatopancreatic necrosis disease in shrimp in the Philippines. Microbiol Resour Announc 8:e01020-19. https://doi.org/10.1128/MRA.01020-19.

                Author information
                https://orcid.org/0000-0002-3351-3465
                Article
                MRA01020-19
                10.1128/MRA.01020-19
                6856269
                31727703
                41119a3f-aaaa-4c72-b62c-c970a2eba9fc
                Copyright © 2019 Penir et al.

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                : 6 September 2019
                : 21 October 2019
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 7, Pages: 2, Words: 1098
                Funding
                Funded by: Department of Science and Technology (DOST), https://doi.org/10.13039/501100010218;
                Award ID: Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARD)
                Award Recipient :
                Categories
                Genome Sequences
                Custom metadata
                November 2019

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