Enhanced synthesis of andrographolide by Aspergillus niger and Penicillium expansum elicitors in cell suspension culture of Andrographis paniculata (Burm. f.) Nees

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

Andrographis paniculata (Burm. f.) Nees is an important medicinal plant which has enormous applications in pharmaceutical industries. Cell suspension culture of Andrographis paniculata (Burm. f.) Nees. was treated with Aspergillus niger and Penicillium expansum elicitors to enhance the synthesis of andrographolide, the bioactive constituent of A. paniculata.

Result

The elicitation treatment with fungal elicitors ( A. niger and P. expansum) was observed to be most suitable for eliciting andrographolide production in the culture. The quantification of andrographolide was done using High Performance Liquid Chromatography (HPLC) technique. A. niger extract (1.5 ml with10 days treatment duration) revealed 6.94 fold increase in andrographolide content (132 μg) which was higher than the control (19 μg). P. expansum elicitor (0.6% with 8 days treatment duration) could reveal 6.23 fold enhancement in andrographolide content (81.0 μg) over control (13 μg).

Conclusion

The results obtained reveal that the longer treatment duration is most favorable for the elicitation of andrographolide using both the fungal elicitors.

Electronic supplementary material

The online version of this article (doi:10.1186/1999-3110-54-49) contains supplementary material, which is available to authorized users.

Related collections

Most cited references 37

Record: found
Abstract: found
Article: not found

Plants as source of drugs.

S.M.K. Rates (2001)

This work presents a study of the importance of natural products, especially those derived from higher plants, in terms of drug development. It describes the main strategies for obtaining drugs from natural sources, fields of knowledge involved, difficulties and perspectives. It also includes a brief discussion of the specific situation in Brazil regarding the use of, trade in, and research into therapeutic resources of natural origin and the general lack of awareness of the use of potentially toxic plants, mainly in folk medicine.

0 comments Cited 297 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

A phase I trial of andrographolide in HIV positive patients and normal volunteers.

C Calabrese, Daniel Berman, Cynthia A. Wenner … (2000)

A phase I dose-escalating clinical trial of andrographolide from Andrographis paniculata was conducted in 13 HIV positive patients and five HIV uninfected, healthy volunteers. The objectives were primarily to assess safety and tolerability and secondarily to assess effects on plasma virion HIV-1 RNA levels and CD4(+) lymphocyte levels. No subjects used antiretroviral medications during the trial. Those with liver or renal abnormalities were excluded. The planned regimen was 5 mg/kg bodyweight for 3 weeks, escalating to 10 mg/kg bodyweight for 3 weeks, and to 20 mg/kg bodyweight for a final 3 weeks. The trial was interrupted at 6 weeks due to adverse events including an anaphylactic reaction in one patient. All adverse events had resolved by the end of observation. A significant rise in the mean CD4(+) lymphocyte level of HIV subjects occurred after administration of 10 mg/kg andrographolide (from a baseline of 405 cells/mm(3) to 501 cells/mm(3); p = 0.002). There were no statistically significant changes in mean plasma HIV-1 RNA levels throughout the trial. Andrographolide may inhibit HIV-induced cell cycle dysregulation, leading to a rise in CD4(+) lymphocyte levels in HIV-1 infected individuals. Copyright 2000 John Wiley & Sons, Ltd.

0 comments Cited 92 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses.

D Scheel-Toellner, T Nürnberger, T Jabs … (1994)

An oligopeptide of 13 amino acids (Pep-13) identified within a 42 kDa glycoprotein elicitor from P. mega-sperma was shown to be necessary and sufficient to stimulate a complex defense response in parsley cells comprising H+/Ca2+ influxes, K+/Cl- effluxes, an oxidative burst, defense-related gene activation, and phytoalexin formation. Binding of radiolabeled Pep-13 to parsley microsomes and protoplasts was specific, reversible, and saturable. Identical structural features of Pep-13 were found to be responsible for specific binding and initiation of all plant responses analyzed. The high affinity binding site recognizing the peptide ligand (KD = 2.4 nM) may therefore represent a novel class of receptors in plants, and the rapidly induced ion fluxes may constitute elements of the signal transduction cascade triggering pathogen defense in plants.

0 comments Cited 78 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Moinuddin M A Vakil: m.mvakil@yahoo.com

Vijay D Mendhulkar: m.mvakil@yahoo.com

Journal

Journal ID (nlm-ta): Bot Stud

Journal ID (iso-abbrev): Bot Stud

Title: Botanical Studies

Publisher: Springer Berlin Heidelberg (Berlin/Heidelberg )

ISSN (Print): 1817-406X

ISSN (Electronic): 1999-3110

Publication date (Electronic): 24 October 2013

Publication date PMC-release: 24 October 2013

Publication date Collection: December 2013

Volume: 54

Electronic Location Identifier: 49

Affiliations

GRID grid.44871.3e, ISNI 0000000106680201, Department of Botany, , The Institute of Science, ; 15, Madam Cama Road, Mumbai, 4000 32 India

Article

Publisher ID: 43

DOI: 10.1186/1999-3110-54-49

PMC ID: 5430361

PubMed ID: 28510886

SO-VID: 42081bf4-3770-4f1d-8e47-75162023fd98

License:

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

History

Date received : 16 September 2013

Date accepted : 10 October 2013

Custom metadata

Keywords: active constituents,fungal elicitors,hplc analysis,secondary metabolites,tissue culture techniques

Data availability:

Keywords: active constituents, fungal elicitors, hplc analysis, secondary metabolites, tissue culture techniques

Enhanced synthesis of andrographolide by Aspergillus niger and Penicillium expansum elicitors in cell suspension culture of Andrographis paniculata (Burm. f.) Nees

Read this article at

Abstract

Background

Result

Conclusion

Electronic supplementary material

Related collections

Inflamed lung in vitro co-culture models

Most cited references 37

Plants as source of drugs.

A phase I trial of andrographolide in HIV positive patients and normal volunteers.

High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses.

Author and article information

Contributors

Journal

Affiliations

Article

History

Categories

Custom metadata

Comments

Comment on this article

Similar content 370

Cited by 3

Most referenced authors 610