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      Successful development of novel monoclonal antibodies against HTLV-1 bZIP factor and their applications in studying the pathogenesis of HAM/TSP

      abstract
      1 , , 1 , 1 , 2 , 3 , 1
      Retrovirology
      BioMed Central
      15th International Conference on Human Retroviruses: HTLV and Related Viruses
      5-8 June 2011

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          Abstract

          Background A recent report has indicated that transgenic expression of HTLV-1 bZIP factor (HBZ) in CD4+ T cells induced T-cell lymphomas and systemic inflammation in mice. However, there is limited information on in vivo expression of HBZ in HTLV-1 infected individuals especially HAM/TSP. Methods We have generated rat and human monoclonal antibodies against HBZ by hybridoma methodology and a phage display system, and developed the ELISA system for the detection of HBZ protein and anti-HBZ antibodies. Using these reagents, we analyzed the expression of HBZ protein in plasma and peripheral blood mononuclear cells (PBMCs) of HTLV-1 infected individuals as well as HTLV-1 infected cell lines. Then the results were compared with the real time PCR data. Results Although we successfully detected HBZ protein in an enforced overexpressed cells and HTLV-1 infected cell lines by flow cytometry, ELISA, immunofluorescence and immunoblotting, we could not detect HBZ protein or anti-HBZ antibodies in HAM/TSP patients and asymptomatic carrires (ACs) by our system. In contrast, HBZ mRNA was significantly elevated in HAM/TSP patients than ACs. Especially, rapidly progressive HAM/TSP patients showed extremely high expression of HBZ mRNA but not tax mRNA in their PBMCs. Conclusions These findings suggest that although expression of HBZ protein was suppressed in vivo in HAM/TSP patients and ACs, high expression of HBZ mRNA is closely associated with the pathogenesis of HAM/TSP.

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          Author and article information

          Conference
          Retrovirology
          Retrovirology
          BioMed Central
          1742-4690
          2011
          6 June 2011
          : 8
          : Suppl 1
          : A244
          Affiliations
          [1 ]Department of Immunology, University of the Ryukyus, Okinawa, 903-0215, Japan
          [2 ]Department of Neurology, Ohkatsu Hospital, Kagoshima, 890-0067, Japan
          [3 ]Department of Neurology, National Okinawa Hospital, Okinawa, 901-2214, Japan
          Article
          1742-4690-8-S1-A244
          10.1186/1742-4690-8-S1-A244
          3112725
          4232f10c-dd86-49ea-891b-8d78b1118f8c
          Copyright ©2011 Saito et al; licensee BioMed Central Ltd.

          This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

          15th International Conference on Human Retroviruses: HTLV and Related Viruses
          Leuven and Gembloux, Belgium
          5-8 June 2011
          History
          Categories
          Meeting Abstract

          Microbiology & Virology
          Microbiology & Virology

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