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      PRL-3和RhoC在A549细胞中的表达及意义 Translated title: Expressions and Signifcances of PRL-3 and RhoC in A549 Cell

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          Abstract

          背景与目的

          在前期研究中,我们发现在非小细胞肺癌中PRL-3和RhoC的表达具有相关性,提示两者可能存在相互作用。本研究通过检测两者在A549细胞迁移中的作用以及是否存在相互作用,为进一步研究它们在肿瘤发生发展中的作用机理提供实验依据。

          方法

          应用PRL-3抗体、RhoC抗体分别阻断A549细胞中PRL-3和RhoC的功能,采用细胞划痕实验检测两者对其迁移能力的影响;应用RT-PCR检测PRL-3和RhoC表达的变化。

          结果

          PRL-3和RhoC功能被阻断后,A549细胞迁移能力明显降低;阻断PRL-3的A549细胞中RhoC表达降低,然而阻断RhoC的A549细胞中PRL-3表达无明显改变。

          结论

          PRL-3和RhoC可能具有促进A549细胞远处转移的功能;PRL-3可能具有上调RhoC表达的功能;PRL-3促进癌细胞远处转移的功能可能是通过RhoC及其下游因子实现的,而RhoC对PRL-3的表达可能无反馈性调节作用。

          Translated abstract

          Background and objective

          The expression of phosphatase of regenerating liver-3 (PRL-3) is cor- related with Ras homologue C (RhoC) in non-small cell lung cancer (NSCLC), suggesting that they have interactions. The aim of this study is to investigate the functions of PRL-3 and RhoC in the migration of A549 cell and the potential mechanism of PRL-3 and RhoC in carcinogenesis and cancer development.

          Methods

          PRL-3Ab and RhoCAb were used to block the functions of PRL-3 and RhoC respectively. Wound healing assay was applied to detect the migration of A549 cell and the ex- pression levels of PRL-3 and RhoC were detected by RT-PCR.

          Results

          The migration of A549 cell decreased after blockage of PRL-3 and RhoC. The expression of RhoC decreased when PRL-3 was blocked without any changes on the expression of PRL-3.

          Conclusion

          PRL-3, RhoC could increase cell migration in A549 cells.

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          Most cited references 16

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          Genomic analysis of metastasis reveals an essential role for RhoC.

          The most damaging change during cancer progression is the switch from a locally growing tumour to a metastatic killer. This switch is believed to involve numerous alterations that allow tumour cells to complete the complex series of events needed for metastasis. Relatively few genes have been implicated in these events. Here we use an in vivo selection scheme to select highly metastatic melanoma cells. By analysing these cells on DNA arrays, we define a pattern of gene expression that correlates with progression to a metastatic phenotype. In particular, we show enhanced expression of several genes involved in extracellular matrix assembly and of a second set of genes that regulate, either directly or indirectly, the actin-based cytoskeleton. One of these, the small GTPase RhoC, enhances metastasis when overexpressed, whereas a dominant-negative Rho inhibits metastasis. Analysis of the phenotype of cells expressing dominant-negative Rho or RhoC indicates that RhoC is important in tumour cell invasion. The genomic approach allows us to identify families of genes involved in a process, not just single genes, and can indicate which molecular and cellular events might be important in complex biological processes such as metastasis.
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            A phosphatase associated with metastasis of colorectal cancer.

            To gain insights into the molecular basis for metastasis, we compared the global gene expression profile of metastatic colorectal cancer with that of primary cancers, benign colorectal tumors, and normal colorectal epithelium. Among the genes identified, the PRL-3 protein tyrosine phosphatase gene was of particular interest. It was expressed at high levels in each of 18 cancer metastases studied but at lower levels in nonmetastatic tumors and normal colorectal epithelium. In 3 of 12 metastases examined, multiple copies of the PRL-3 gene were found within a small amplicon located at chromosome 8q24.3. These data suggest that the PRL-3 gene is important for colorectal cancer metastasis and provide a new therapeutic target for these intractable lesions.
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              Up-regulation of small GTPases, RhoA and RhoC, is associated with tumor progression in ovarian carcinoma.

              To clarify the role of small GTPases Rho in the biologic behavior of ovarian carcinoma, we first examined the mRNA expression of RhoA, RhoB, and RhoC in benign, borderline, and malignant ovarian tumors using RT-PCR and real-time RT-PCR. The expression and localization of RhoA protein were also analyzed by Western blotting and immunohistochemistry. Finally, we examined whether up-regulation of Rho enhances the invasiveness of ovarian cancer cells in vitro. Analysis of mRNA levels of the Rho family genes revealed that levels of both RhoA and RhoC were significantly higher in carcinomas than in benign tumors (RhoA, p = 0.0035; RhoC, p = 0.0006). According to histologic subtype, both RhoA and RhoC mRNA levels in serous carcinomas were significantly higher than those in other histologic types. With regard to the International Federation of Gynecological and Obstetrics stage classification, both of RhoA and RhoC mRNA levels were significantly higher in tumors of Stages III+IV than in those of Stages I+II (RhoA, p = 0.0200; RhoC, p = 0.0057). In addition, analysis of matched pairs of primary and disseminated lesions demonstrated that expression of both RhoA and RhoC mRNA was significantly higher in metastatic than in primary tumors. Examination of the protein level showed that expression of RhoA was also increased in advanced ovarian carcinomas, especially those of serous histology. Accordingly, we hypothesized that up-regulation of Rho GTPases plays an important role in the progression of ovarian carcinoma. Matrigel invasion assay using the ovarian cancer cell line, SKOV3, showed that up-regulation and activation after treatment with lysophosphatidic acid was associated with enhanced invasion of the cancer cells. This increase in invasiveness was suppressed by the addition of C3, a specific inhibitor of Rho. These findings suggest that up-regulation of Rho GTPases is important in the tumor progression of ovarian carcinoma and that Rho family proteins could be a molecular target in cancer therapy.
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                Author and article information

                Contributors
                Journal
                Zhongguo Fei Ai Za Zhi
                Zhongguo Fei Ai Za Zhi
                ZGFAZZ
                Chinese Journal of Lung Cancer
                中国肺癌杂志编辑部 (天津市和平区南京路228号300020 )
                1009-3419
                1999-6187
                20 December 2010
                : 13
                : 12
                : 1160-1164
                Affiliations
                [1 ] 710038 西安,第四军医大学唐都医院胸外科 Department of Toracic Surgery, Tangdu Hospital, the Fourth Military Medical University, Xi'an 710038, China
                [2 ] 810001 西宁,青海大学附属医院肾内科 Department of Nephrology, Qinghai University Afliated Hospital, Xi'ning 810001, China
                Author notes
                王小平, Xiaoping WANG, E-mail: wxpchest@ 123456fmmu.edu.cn
                Article
                zgfazz-13-12-1160 R734.2
                10.3779/j.issn.1009-3419.2010.12.15
                6426730
                21159255
                版权所有©《中国肺癌杂志》编辑部2010Copyright ©2010 Chinese Journal of Lung Cancer. All rights reserved.

                This is an open access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) License. See: https://creativecommons.org/licenses/by/3.0/

                Categories
                短篇报道
                Short Communication

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