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      Biological and serological characteristics of Zucchini yellow mosaic virus isolated from Zucchini (Cucurbita pepo), squash (C. maxima), and pumpkin (C. moschata) Translated title: Características biológicas y serológicas del virus mosaico amarillo del calabacín aislado de calabacín (Cucurbita pepo), calabaza de invierno (C. maxima) y calabaza (C. moschata)


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          Abstract Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses of cucurbit crops, causing severe mosaic, blistering of leaf lamina, malformation, and knobbed fruits, and presents an important biological variability. The objective of this study was to determine the prevalence and incidence of ZYMV in major summer (Cucurbita pepo), and winter squashes (C. maxima, and C. moschata) growing areas of Turkey-Samsun. Field surveys were made in 2015-2016 cropping seasons in major cucurbit growing regions. In five regions, 225 symptomatic samples were collected. Samples from three different species of Cucurbita were tested by biological and serological method using ZYMV polyclonal antiserum. Serological test results revealed that 84 out of 225 samples contained ZYMV, 34 out of 70 zucchini (C. pepo) samples, 28 out of 90 squash (C. maxima) samples containing ZYMV, and 22 out of 65 pumpkin (C. moschata) samples had ZYMV in 2015-2016. Three ZYMV isolates were obtained from zucchini at Carsamba (ZYMV-CA), winter squash at Tekkekoy (ZYMV-TE), and pumpkin at Bafra (ZYMV-BA), and their biological variability was detected on different hosts, including cucurbit crops.

          Translated abstract

          Resumen El virus del mosaico amarillo del calabacín (ZYMV) es uno de los virus de mayor importancia económica para los cultivos de cucurbitáceas, que causa mosaico severo, formación de ampollas en la lámina foliar, malformación y frutos nudosos, y presenta una importante variabilidad biológica. El objetivo de este estudio fue determinar la prevalencia e incidencia del ZYMV en las principales áreas de cultivo de verano (Cucurbita pepo) y calabazas de invierno (C. maxima y C. moschata) en zonas de cultivo en Turquía-Samsun.Los estudios de campo se realizaron en las temporadas de cultivo de 2015-2016 en las principales regiones de cultivo de cucurbitáceas. En cinco regiones, se recolectaron 225 muestras sintomáticas. Las muestras de tres especies diferentes de Cucurbita se probaron mediante un método biológico y serológico utilizando el antisuero policlonal ZYMV. Los resultados de las pruebas serológicas revelaron que 84 de 225 muestras contenían ZYMV: 34 de 70 muestras de zucchini (C. pepo), 28 de 90 muestras de calabaza (C. maxima) y 22 de las 65 muestras de calabazas (C. moschata) en 2015-2016. Se obtuvieron tres aislados de ZYMV de calabacín en Carsamba (ZYMV-CA), calabaza de invierno en Tekkekoy (ZYMV-TE) y calabaza en Bafra (ZYMV-BA), y su variabilidad biológica se detectó en diferentes huéspedes, incluyendo los cultivos de cucurbitáceas.

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          Most cited references15

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          Virus infections in wild plant populations are both frequent and often unapparent.

          Pathogens are thought to regulate host populations. In agricultural crops, virus infection reduces yield. However, in wild plants little is known about the spatial and temporal patterns of virus prevalence. Thus, pathogen effects on plant population dynamics are unclear. Prevalence data provide necessary background for (1) evaluating the effects of virus infection on plant population size and dynamics and (2) improving risk assessment of virus-resistant transgenic crops. We used ELISA and RT-PCR to survey wild Cucurbita pepo populations over 4 years for five viruses, aphid-transmitted viruses of the genus Potyvirus as a group and PCR to survey for virus-resistance transgenes. In addition, we surveyed the literature for reports of virus prevalence in wild populations. In 21 C. pepo populations, virus prevalence (0-74%) varied greatly among populations, years, and virus species. In samples analyzed by both ELISA and RT-PCR, RT-PCR detected 6-44% more viruses than did ELISA. Eighty percent of these infections did not cause any visually apparent symptoms. In our samples, the virus-resistance transgene was not present. In 30 published studies, 92 of 146 tested species were infected with virus, and infection rates ranged from 0.01-100%. Most published studies used ELISA, suggesting virus prevalence is higher than reported. In wild C. pepo, the demographic effects of virus are likely highly variable in space and time. Further, our literature survey suggests that such variation is probably common across plant species. Our results indicate that risk assessments for virus-resistant transgenic crops should not rely on visual symptoms or ELISA and should include data from multiple populations over multiple years.
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            Aphid Attraction and Preference for Soybean and Pepper Plants Infected with Potyviridae

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              The Genes of Pumpkin and Squash

              Pumpkin and squash ( Cucurbita L. spp.) are important cucurbit crops and are grown in almost all arable regions of the world. The three economically important species, Cucurbita pepo L., Cucurbita moschata Duchesne, and Cucurbita maxima Duchesne are highly polymorphic in fruit characteristics, inspiring much research into their inheritance. A comprehensive list of genes for Cucurbita was last published more than a decade ago. This new gene list for pumpkin and squash includes descriptions of gene interactions and the genetic background of the parents that had been used for crossing to allow easy confirmation of previous work and provide a sound foundation for further investigation. This gene list includes 79 loci for phenotypic/morphological traits and 48 polymorphic allozyme loci. Linkage and mapping are discussed.

                Author and article information

                Role: ND
                Colegio de Postgraduados (México, DF, Mexico )
                November 2018
                : 52
                : 7
                : 1005-1011
                [1] Samsun orgnameOndokuz Mayis University orgdiv1Faculty of Agriculture orgdiv2Department of Plant Protection Turkey

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 16, Pages: 7

                SciELO Mexico

                Plant protection

                calabaza de invierno,ZYMV,winter squash,virus,calabaza de verano,pruebas biológicas y serológicas,summer squash,Biological and serological tests


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