8
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Unexpectedly complex editing patterns at dinucleotide insertion sites in Physarum mitochondria.

      Molecular and Cellular Biology
      Animals, Base Sequence, DNA, Protozoan, genetics, Mitochondria, metabolism, Physarum polycephalum, RNA Editing, RNA, Messenger, RNA, Protozoan, Ribonucleotides

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Many of the RNAs transcribed from the mitochondrial genome of Physarum polycephalum are edited by the insertion of nonencoded nucleotides, which are added either singly or as dinucleotides. In addition, at least one mRNA is also subject to substitutional editing in which encoded C residues are changed to U residues posttranscriptionally. We have shown previously that the predominant type of editing in these organelles, the insertion of nonencoded single C residues, occurs cotranscriptionally at the growing end of the RNA chain. However, less is known about the timing of dinucleotide addition, and it has been suggested that these insertions occur at a later stage in RNA maturation. Here we examine the addition of both single nucleotides and dinucleotides into nascent RNAs synthesized in vitro and in vivo. The distribution of added nucleotides within individual cloned cDNAs supports the hypothesis that all insertion sites are processed at the same time relative to transcription. In addition, the patterns of partial editing and misediting observed within these nascent RNAs suggest that separate factors may be required at a subset of dinucleotide insertion sites and raise the possibility that in vivo, nucleotides may be added to RNA and then changed posttranscriptionally.

          Related collections

          Author and article information

          Journal
          15340046
          515060
          10.1128/MCB.24.18.7821-7828.2004

          Chemistry
          Animals,Base Sequence,DNA, Protozoan,genetics,Mitochondria,metabolism,Physarum polycephalum,RNA Editing,RNA, Messenger,RNA, Protozoan,Ribonucleotides

          Comments

          Comment on this article