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      Effects of Fasting and Refeeding on Antral, Duodenal, and Serum Gastrin Levels and on Colonic Thymidine Kinase Activity in Rat

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          Abstract

          Antral, duodenal, and serum gastrin levels and colonic thymidine kinase activity were determined in 1- to 4-day-fasted rats and after refeeding of 4-day-fasted rats for 3–24 h. The effect of pentagastrin on colonic thymidine kinase activity was also determined. Total deprivation of food caused a drastic reduction in gastrin concentrations in serum and tissues. After 4 days of fasting, serum gastrin levels in most animals fell below the present detection limit of the assay (10–15 pg/ml), and antral and duodenal gastrin levels decreased to 15 and 50% of the respective initial fed control. After 9 and 24 h of refeeding, gastrin concentration in serum and antrum had increased to about 35% of the initial fed level. On the other hand, refeeding for 3–24 h produced no significant change in duodenal gastrin concentration. Fasting for 1–4 days resulted in a 60–70% reduction in colonic thymidine kinase activity, compared to the initial fed control. Refeeding caused a prompt stimulation in the enzyme activity, which after 6 h was found to be 72% above the 4-day-fasted group. Daily injection of pentagastrin, at doses between 125 and 500 µg/kg, during a 4-day fasting period resulted in a significant stimulation in colonic thymidine kinase activity, compared to the saline-treated control. The maximal stimulation of an enzyme activity 90% higher than in the saline control was attained with a pentagastrin dose of 125 µg/kg. Higher doses decreased the maximal stimulatory effect of pentagastrin. In view of the observation that following refeeding colonic thymidine kinase activity was stimulated long before gastrin levels in serum or tissues were increased, we conclude that the initial postprandial rise in colonic cellular proliferative activity is independent of gastrin.

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          Author and article information

          Journal
          HRE
          10.1159/issn.0018-5051
          Hormone Research in Paediatrics
          S. Karger AG
          0018-5051
          2571-6603
          1984
          1984
          26 November 2008
          : 19
          : 2
          : 127-134
          Affiliations
          Institute of Medical Biochemistry, University of Aarhus, Denmark
          Article
          179877 Horm Res 1984;19:127–134
          10.1159/000179877
          © 1984 S. Karger AG, Basel

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          Page count
          Pages: 8
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