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Abstract
In an attempt to develop a label- and reagent-free electrochemical method for the
detection of lectin-glycoprotein interactions, we tested lectin-concanavalin A (ConA),
glycoprotein-ovalbumin (Ova) and their complex using chronopotentiometric stripping
(CPS) analysis and a hanging mercury drop electrode. Incubation of ConA with Ova resulted
in an increase of the CPS peak H of the complex as compared to the CPS peaks of individual
Ova and ConA proteins. Qualitatively similar results were obtained with other glycoprotein-lectin
couples (ConA-RNase B and lectin from Sambucus nigra-fetuin). Using the CPS method,
we were able to follow the course of complex formation in solution. Comparable responses
of Ova, ConA and ConA-Ova complex were obtained not only at the mercury electrode
but also with solid amalgam electrodes, which are more suitable for parallel analysis.
It can be anticipated that electrochemical methods, namely CPS, will find application
in glycomics and proteomics.