6
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      TRPA1 mediates the cardiac effects of acrolein through parasympathetic dominance but also sympathetic modulation in mice

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Numerous studies have demonstrated that short-term air pollution exposure causes cardiac autonomic imbalance as measured by heart rate variability (HRV). We previously showed that a single exposure to acrolein, a ubiquitous gaseous component of air pollution, not only causes autonomic imbalance, but also increases arrhythmia through transient receptor potential A1 (TRPA1) cation channels. Thus, the goal of this study was to characterize acrolein-induced autonomic changes in both normal and TRPA1-knockout mice (KO). Conscious, unrestrained C57BL/6 (WT) and KO mice were exposed to 3 ppm acrolein for 3 hours. Separate groups were treated with either atenolol (sympathetic blocker), atropine (parasympathetic blocker) or hexamethonium (autonomic neurotransmission blocker), immediately before exposure. Electrocardiogram (ECG) and heart rate (HR) were recorded continuously before, during and after exposure. Exposure to acrolein produced significant increases in standard deviation of normal-to-normal R-R intervals (SDNN), Root Mean Square of the Successive Differences (RMSSD) and Low-Frequency (LF), as well as an increase in arrhythmia in WT mice. Treatment with atenolol reduced this response while atropine enhanced it, and both drugs blocked the acrolein-induced increase in arrhythmia; hexamethonium had no effect. On the other hand, neither acrolein nor any drug had an effect in the KO mice. Thus, acrolein-induced HRV responses appear to be mediated by a combined parasympathetic and sympathetic modulation. KO mice did not demonstrate any increases in HRV with exposure to acrolein. These data demonstrate that the cardiac effects of irritant air pollutants likely involve disruption of homeostatic balance and altered regulation even in healthy animals.

          Related collections

          Author and article information

          Journal
          0416575
          7769
          Toxicol Appl Pharmacol
          Toxicol. Appl. Pharmacol.
          Toxicology and applied pharmacology
          0041-008X
          1096-0333
          19 October 2018
          05 April 2018
          15 May 2018
          15 May 2019
          : 347
          : 104-114
          Affiliations
          [1 ]Curriciulm in Toxicology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
          [2 ]Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711
          Author notes
          Corresponding author: Mehdi S. Hazari, Environmental Public Health Division, USEPA, 109 Alexander Drive, B105; Research Triangle Park, NC 27711; (Phone: 919-541-4588; Fax: 919-541-0034; hazari.mehdi@ 123456epa.gov )
          Article
          PMC6220342 PMC6220342 6220342 epapa1507801
          10.1016/j.taap.2018.03.027
          6220342
          29627347
          Categories
          Article

          Comments

          Comment on this article