PET imaging of the 18 kDa translocator protein (TSPO), a biomarker of microglial activity,
receives growing interest in clinical and preclinical applications of neuroinflammatory
and neurodegenerative brain diseases. In globally affected brains, intra-cerebral
pseudo reference regions are not feasible. Consequently, many brain-independent approaches
have been attempted, including SUV analysis and normalization to muscle- or heart
uptake, aiming to stabilize quantitative analysis. In this study, we systematically
compared different image normalization methods for static late phase TSPO-PET imaging
of rodent brain.