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      Systematic artifacts in metagenomes from complex microbial communities.

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          Abstract

          Metagenomics is providing an unprecedented view of the taxonomic diversity, metabolic potential and ecological role of microbial communities in biomes as diverse as the mammalian gastrointestinal tract, the marine water column and soils. However, we have found a systematic error in metagenomes generated by 454-based pyrosequencing that leads to an overestimation of gene and taxon abundance; between 11% and 35% of sequences in a typical metagenome are artificial replicates. Here we document the error in several published and original datasets and offer a web-based solution (http://microbiomes.msu.edu/replicates) for identifying and removing these artifacts.

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          Author and article information

          Journal
          ISME J
          The ISME journal
          Springer Science and Business Media LLC
          1751-7370
          1751-7362
          Nov 2009
          : 3
          : 11
          Affiliations
          [1 ] Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA.
          Article
          ismej200972
          10.1038/ismej.2009.72
          19587772
          44cc26f0-a234-4233-836f-f59791e431ea
          History

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