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      Semiconductor nanocrystals as fluorescent biological labels.

      Science (New York, N.Y.)

      3T3 Cells, Actins, Animals, Biotinylation, Cadmium Compounds, Cell Nucleus, ultrastructure, Crystallization, Cytoskeleton, Fluorescent Dyes, Mice, Microscopy, Confocal, Microscopy, Fluorescence, Molecular Probe Techniques, Selenium Compounds, Semiconductors, Silicon Dioxide, Solubility, Spectrometry, Fluorescence, Staining and Labeling, methods, Sulfides

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          Abstract

          Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitation spectrum were demonstrated in a dual-emission, single-excitation labeling experiment on mouse fibroblasts. These nanocrystal probes are thus complementary and in some cases may be superior to existing fluorophores.

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          9748157

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