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      Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices

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          Abstract

          Antimicrobial resistance (AMR) is recognized as a global threat to human health. Rapid detection and characterization of AMR is a critical component of most antibiotic stewardship programs. Methods based on amplification of nucleic acids for detection of AMR are generally faster than culture-based approaches but they still require several hours to more than a day due to the need for transporting the sample to a centralized laboratory, processing of sample, and sometimes DNA purification and concentration. Nucleic acids-based point-of-care (POC) devices are capable of rapidly diagnosing antibiotic-resistant infections which may help in making timely and correct treatment decisions. However, for most POC platforms, sample processing for nucleic acids extraction and purification is also generally required prior to amplification. Direct amplification, an emerging possibility for a number of polymerases, has the potential to eliminate these steps without significantly impacting diagnostic performance. This review summarizes direct amplification methods and their implication for rapid measurement of AMR. Future research directions that may further strengthen the possibility of integrating direct amplification methods with POC devices are also summarized.

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          Author and article information

          Journal
          101519733
          38347
          Anal Methods
          Anal Methods
          Analytical methods : advancing methods and applications
          1759-9660
          1759-9679
          25 March 2018
          31 January 2017
          28 February 2017
          13 April 2018
          : 9
          : 8
          : 1229-1241
          Affiliations
          [a ]Department of Civil and Environmental Engineering, Michigan State University, East Lansing, MI 48824, USA
          [b ]Pediatrics and Human Development, Michigan State University, East Lansing, MI 48824, USA
          [c ]Department of Microbiology, Sparrow Laboratories, Sparrow Health System, Lansing, MI 48912, USA
          [d ]Osteopathic Medical Specialties, Section of Emergency Medicine, Michigan State University, East Lansing, MI 4882, USA
          [e ]Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA
          [f ]Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USA
          Article
          PMC5898395 PMC5898395 5898395 nihpa954181
          10.1039/C6AY03405E
          5898395
          29657581
          451749eb-18e6-4492-b03c-8b3f2da4fc39
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