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      Fast scanning coaxial optoacoustic microscopy

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          Abstract

          The hybrid nature of optoacoustic imaging might impose limitations on concurrent placement of optical and ultrasonic detection components, especially in high resolution microscopic applications that require dense arrangements and miniaturization of components. This hinders optimal deployment of the optical excitation and ultrasonic detection paths, leading to reduction of imaging speed and spatial resolution performance. We suggest a compact coaxial design for optoacoustic microscopy that allows optimizing both the light illumination and ultrasonic detection parameters of the imaging system. System performance is showcased in phantoms and in vivo imaging of microvasculature, achieving real time operation in two dimensions and penetration of 6 mm into optically dense human tissues.

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          Most cited references 13

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          Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo

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            Volumetric real-time multispectral optoacoustic tomography of biomarkers.

            Multispectral optoacoustic tomography (MSOT) has recently been developed to enable visualization of optical contrast and tissue biomarkers, with resolution and speed representative of ultrasound. In the implementation described here, MSOT enables operation in real-time mode by capturing single cross-sectional images in <1 ms from living small animals (e.g., mice) and other tissues of similar dimensions. At the core of the method is illumination of the object using multiple wavelengths in order to resolve spectrally distinct biomarkers over background tissue chromophores. The system allows horizontal placement of a mouse in the imaging chamber and three-dimensional scanning of the entire body without the need to immerse the mouse in water. Here we provide a detailed description of the MSOT scanner components, system calibration, selection of image reconstruction algorithms and animal handling. Overall, the entire protocol can be completed within 15-30 min for acquisition of a whole-body multispectral data set from a living mouse.
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              In vivo high-resolution 3D photoacoustic imaging of superficial vascular anatomy.

              The application of a photoacoustic imaging instrument based upon a Fabry-Perot polymer film ultrasound sensor to imaging the superficial vasculature is described. This approach provides a backward mode-sensing configuration that has the potential to overcome the limitations of current piezoelectric based detection systems used in superficial photoacoustic imaging. The system has been evaluated by obtaining non-invasive images of the vasculature in human and mouse skin as well as mouse models of human colorectal tumours. These studies showed that the system can provide high-resolution 3D images of vascular structures to depths of up to 5 mm. It is considered that this type of instrument may find a role in the clinical assessment of conditions characterized by changes in the vasculature such as skin tumours and superficial soft tissue damage due to burns, wounds or ulceration. It may also find application in the characterization of small animal cancer models where it is important to follow the tumour vasculature over time in order to study its development and/or response to therapy.
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                Author and article information

                Journal
                Biomed Opt Express
                Biomed Opt Express
                BOE
                Biomedical Optics Express
                Optical Society of America
                2156-7085
                26 June 2012
                01 July 2012
                26 June 2012
                : 3
                : 7
                : 1724-1731
                Affiliations
                Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
                Author notes
                Article
                167251
                10.1364/BOE.3.001724
                3395494
                22808441
                © 2012 Optical Society of America

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

                Funding
                Funded by: ERC Starting Independent Researcher Grant
                Categories
                Photoacoustic Imaging and Spectroscopy
                Custom metadata
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