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      HER2 amplification and overexpression are significantly correlated in mucinous epithelial ovarian cancer.

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          Abstract

          HER2 gene amplification and protein over-expression are important factors in predicting clinical sensitivity to anti-HER2 therapies in breast, gastric or gastroesophageal junction cancer patients. The aim of this study was to evaluate the correlation between HER2 gene copy numbers and HER2 protein expressions in mucinous epithelial ovarian cancer (EOC). Of the 49 tissue microarray samples of mucinous EOC, we applied 2010 ToGA trial (Trastuzumab for Gastric Cancer) surgical specimen scoring criteria to analyze the HER2 protein expression by an immunohistochemistry (IHC) test with Dako (Carpenteria, CA), c-erb-B2 antibody, and the HER2 gene amplification by the fluorescence in situ hybridization (FISH) test with Abbott/Vysis PathVysion HER2 DNA Probe Kit (Abbott Molecular Inc., Des Plaines, IA). We achieved a high overall concordance of 97.56% between nonequivocal HER2 results by IHC and FISH tests. In addition, HER2 gene copies before chromosome-17 correction increased significantly in a stepwise order through the negative, equivocal and positive IHC result categories (P<.001), as did the HER2 gene copies after chromosome-17 correction (P<.001). On the other hand, HER2 IHC results correlated significantly with both chromosome-17-uncorrected HER2 gene copy numbers (ρ=0.630, P<.001) and chromosome-17 corrected HER2 gene copy numbers (ρ=0.558, P<.001). We concluded that both chromosome-17 corrected and uncorrected HER2 gene copies correlated significantly with HER2 IHC results. Tests for the HER2 gene copies per tumor cell either before or after correction of chromosome-17 can be applied as a potentially valuable tool to analyze the HER2 status in mucinous EOC.

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          Author and article information

          Journal
          Hum. Pathol.
          Human pathology
          1532-8392
          0046-8177
          Apr 2014
          : 45
          : 4
          Affiliations
          [1 ] Institute of Medicine, Chung-Shan Medical University, Taichung, Taiwan; Department of Pathology, Chung-Shan Medical University and Chung Shan Medical University Hospital, Taichung, Taiwan.
          [2 ] Department of Statistics and Informatics Science, Providence University, Taichung, Taiwan.
          [3 ] Department of Pathology, Chung-Shan Medical University and Chung Shan Medical University Hospital, Taichung, Taiwan.
          [4 ] Department of Pathology, Mackay Memorial Hospital, Taipei, Taiwan.
          [5 ] Department of Respiratory Therapy, Fu-Jen Catholic University, Taipei, Taiwan.
          [6 ] Institute of Medicine, Chung-Shan Medical University, Taichung, Taiwan; Department of Pathology, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan.
          [7 ] Institute of Medicine, Chung-Shan Medical University, Taichung, Taiwan.
          [8 ] Department of Obstetrics and Gynecology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan; Department of Pathology, School of Medicine, Chung-Shan Medical University and Chung-Shan Medical University Hospital, Taichung, Taiwan.. Electronic address: hanhaly@gmail.com.
          Article
          S0046-8177(13)00523-6
          10.1016/j.humpath.2013.11.016
          24656091
          Copyright © 2014 Elsevier Inc. All rights reserved.

          Mucinous epithelial ovarian cancer, HER2, Polysomy-17

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