Background and Aim: Infertility is a side effect of cancer treatment because of chemotherapy
or radiotherapy. Cryopreservation of testicular tissue or spermatogonial stem cells
before cancer treatment and their transplantation may preserve the natural fertility.
Cryopreservation is a damaging process due to free radicals and toxic effect of frozen
solution. The purpose of this paper is to study the antioxidant and antiapoptotic
effects of 3',4'-dihydroxyflavone on the structure of frozen-thawed testicular tissue
of neonatal mouse.
Materials and Methods: Testes of 6-day-old NMRI mice (N = 20) were isolated. Testicles
were randomly divided into four groups: two groups as control groups for quick and
slow freezing-melting process, and two others as treatment groups that underwent quick
and slow freezing-melting with addition of 3',4'-dihydroxyflavone (10µM) in frozen
solution. For the assessment of structural changes and apoptosis in the frozen-thawed
testes, the hematoxylin-eosin staining and tunnel pod kits were used respectively.
In order to analyze the data, we used Kruskal-Wallis Test and Mann-Whitney Test. The
statistical analysis was entirely done by SPSS software.
Results: Histopathological changes and apoptosis rate were significantly decreased
after thawing in both treatment groups in comparison with control groups.
Conclusion: Adding 3',4'-dihydroxyflavone (10µM) to freezing-melting environment and
the use of quick freezing-melting method can reduce the histopathological and apoptotic