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      Effect of Topical Na-Hyaluronan on Hemidesmosome Formation in n-Heptanol-Induced Corneal Injury

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          The present study investigated the effect of Na-hyaluronan (Na-HA) on the hemidesmosome morphogenesis in n-heptanol-induced corneal wounds. Central epithelial wounds were induced in the rabbit cornea by applying a 5.5-mm round filter paper, which was soaked in n-heptanol, for 60 s. 1% Na-HA in phosphate-buffered saline (PBS) or PBS alone were topically administered to the wounded cornea 4 times daily for up to 7 days. Epithelial healing rates during the first 2 days were not altered by Na-HA. The number of hemidesmosomes in the basement membrane of the central cornea was significantly increased in both 3- and 7-day groups after treatment with 1% Na-HA. The results suggest that topically applied 1% Na-HA may enhance the formation of hemidesmosomes during the early healing phase in n-heptanol-induced corneal wounds.

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          The hyaluronate receptor is preferentially expressed on proliferating epithelial cells

           A.M. Alho (corresponding) ,  C.B. Underhill (corresponding) (1989)
          In the present study, we have examined the distribution of the hyaluronate receptor as well as hyaluronate itself in a variety of adult tissues. The hyaluronate receptor was localized with a monoclonal antibody, termed K-3, while hyaluronate was localized using proteolytic fragments of cartilage proteoglycan. Staining with the K-3 monoclonal antibody revealed that the hyaluronate receptor was present in a variety of epithelia including the skin, cheek, tongue, esophagus, vagina, intestines, oviduct, and bladder. However, it was notably absent from epithelial cells of the cornea and stomach as well as from endothelial cells of blood vessels. When present, the hyaluronate receptor was preferentially located in regions of active cell growth, such as in the basal layers of stratified epithelium and at the base of the crypts of Lieberkuhn in intestinal epithelium. A similar phenomenon was observed in cultured 3T3 cells. Cultures of 3T3 cells that were actively proliferating were found to have greater amounts of the receptor than their nonproliferating counterparts. When the various tissues were examined for hyaluronate, it was found to have a widespread distribution, being present in most of the basement membranes and between the cells in stratified epithelium. Indeed, in many cases, the distribution of hyaluronate closely paralleled that of the hyaluronate receptor. These results suggest that the interaction between hyaluronate and its receptor is involved in cell-to-substratum adhesion.
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            Visualization of hyaluronic acid in the anterior segment of rabbit and monkey eyes


              Author and article information

              Ophthalmic Res
              Ophthalmic Research
              S. Karger AG
              April 1998
              20 February 1998
              : 30
              : 2
              : 96-100
              Departments of a Ophthalmology and b Pharmacology, c Division of Cell and Tissue Biology, Medical Research Center, College of Medicine, Ewha Women’s University, and d Department of Pediatrics, Younsei University, Seoul, Korea; e St. Eriks Eye Hospital, Karolinska Institute, Stockholm, Sweden
              55460 Ophthalmic Res 1998;30:96–100
              © 1998 S. Karger AG, Basel

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              Page count
              Figures: 2, Tables: 1, References: 31, Pages: 5
              Original Paper

              Vision sciences, Ophthalmology & Optometry, Pathology

              Hemidesmosomes, N-Heptanol, Cornea, Rabbit, Na-hyaluronan


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