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      Colonization and Probiotic Effect of Metschnikowia sp. C14 in the Intestine of Juvenile Sea Cucumber, Apostichopus japonicus

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          Abstract

          Viable cell count was used to determine whether Metschnikowia sp. C14 can colonize the intestine of juvenile sea cucumber Apostichopus japonicus. Sea cucumber individuals were divided into two groups, which were fed the control diet for 38 days or the C14-supplemented diet at 10 5 cells g −1 diet for 28 days, then the control diet from day 29 to day 38. The number of C14 cells in the intestine of sea cucumber fed the C14-supplemented diet significantly increased from day 7 to day 28, and decreased from day 29 to day 38. Sea cucumber fed with the diet containing C14 showed a significant increase in trypsin activity and lipase activity from day 21 to day 33 compared with the control. Feeding C14 significantly improved the phagocytic activity and respiratory burst in coelomocytes from day 21 to day 35 and from day 14 to day 38, respectively. In addition, there was an obvious enhancement in lysozyme activity (from day 21 to day 38 or day 33), phenoloxidase activity (from day 21 to day 28) and total nitric oxide synthase activity (from day 14 to day 38) in coelomic fluid supernatant and/or coelomocyte cell lysate supernatant compared with the control. There were significant positive correlations between the number of C14 cells colonizing the intestine and trypsin activity of the intestine, lysozyme activity of the coelomic fluid supernatant and coelomocyte lysate supernatant from sea cucumber. These data suggested that the number of C14 cells should be maintained at 10 5 cfu (colony-forming units) g −1 intestine material for the maximum benefit.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          20 December 2019
          01 February 2020
          : 19
          : 1
          : 225-231
          Affiliations
          1Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea of Ministry of Agriculture and Rural Affair, Dalian Ocean University, Dalian 116023, China
          Author notes
          *Corresponding author: MA Yuexin, Tel: 0086-411-84763096, E-mail: mayuexin@ 123456dlou.edu.cn
          Article
          s11802-020-4143-8
          10.1007/s11802-020-4143-8
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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