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      Contributions of in situ microscopy to the current understanding of stone biodeterioration Translated title: Contribuições da microscopia in situ à comprensão atual do biodeterioro das rochas Translated title: Contribuciones de la microscopia in situ a la comprensión actual del biodeterioro de las rocas

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          Abstract

          In situ microscopy consists of simultaneously applying several microscopy techniques without separating the biological component from its habitat. Over the past few years, this strategy has allowed characterization of the biofilms involved in biodeterioration processes affecting stone monuments and has revealed the biogeophysical and biogeochemical impact of the microbiota present. In addition, through in situ microscopy diagnosis, appropriate treatments can be designed to resolve the problems related to microbial colonization of stone monuments.

          Translated abstract

          A microscopia in situ consiste em aplicar simultaneamente várias técnicas de microscopia sem separação dos componentes biológicos de seu hábitat na rocha. Durante os últimos anos, esta estratégia permitiu caracterizar os biofilmes implicados nos processos do biodeterioro que afetam os monumentos de pedra e revelou o impacto biogeofísico e biogeoquímico da microbiota presente. Além disso, o diagnóstico mediante microscopia in situ permite desenhar tratamentos apropriados para resolver os problemas relacionados com a colonização microbiana dos monumentos de pedra.

          Translated abstract

          La microscopia in situ consiste en aplicar simultáneamente varias técnicas de microscopia sin separación de los componentes biológicos de su hábitat en la roca. Durante los últimos años, esta estrategia ha permitido caracterizar las biopelículas implicados en los procesos del biodeterioro que afectan los monumentos de piedra y ha revelado el impacto biogeofísico y biogeoquímico de la microbiota presente. Además, el diagnóstico mediante microscopia in situ permite diseñar tratamientos apropiados para resolver los problemas relacionados con la colonización microbiana de los monumentos de piedra.

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          Most cited references64

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          Biofouling in water systems--cases, causes and countermeasures.

          H Flemming (2002)
          Biofouling is referred to as the unwanted deposition and growth of biofilms. This phenomenon can occur in an extremely wide range of situations, from the colonisation of medical devices to the production of ultra-pure, drinking and process water and the fouling of ship hulls, pipelines and reservoirs. Although biofouling occurs in such different areas, it has a common cause, which is the biofilm. Biofilms are the most successful form of life on Earth and tolerate high amounts of biocides. For a sustainable anti-fouling strategy, an integrated approach is suggested which includes the analysis of the fouling situation, a selection of suitable components from the anti-fouling menu and an effective and representative monitoring of biofilm development.
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            Geobiology of a microbial endolithic community in the Yellowstone geothermal environment.

            The endolithic environment, the pore space of rocks, is a ubiquitous habitat for microorganisms on the Earth and is an important target of the search for life elsewhere in the Solar System. Photosynthetic, endolithic microbial communities commonly inhabit the outer millimetres to centimetres of all rocks exposed to the Earth's surface. In the most extreme terrestrial climates, such as hot and cold deserts, endolithic microorganisms are often the main form of life. The endolithic microhabitat gives protection from intense solar radiation and desiccation, and it provides mineral nutrients, rock moisture and growth surfaces. Here we describe the discovery and identification of the constituents of an extremely acidic (pH 1) endolithic microbial community inhabiting the pore space of rocks in the geothermal environment of Yellowstone National Park, USA. Subjected to silica mineralization, such endolithic communities constitute biomarkers that can become fossilized and potentially preserved in the geological record. Remnants of these communities could serve as biosignatures and provide important clues about ancient life associated with geothermal environments on the Earth or elsewhere in the Solar System.
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              Weathering of rocks and neogenesis of minerals associated with lichen activity

              P Adamo (2000)
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Journal
                im
                International Microbiology
                INT. MICROBIOL.
                Sociedad Española de Microbiología (Madrid )
                1139-6709
                September 2005
                : 8
                : 3
                : 181-188
                Affiliations
                [1 ] Consejo Superior de Investigaciones Científicas Spain
                Article
                S1139-67092005000300005
                16200496
                45c911f0-910f-4cf5-b552-c60f8d49f92d

                http://creativecommons.org/licenses/by/4.0/

                History
                Categories
                BIOTECHNOLOGY & APPLIED MICROBIOLOGY
                MICROBIOLOGY

                Biotechnology,Microbiology & Virology
                biodeterioration,in situ microscopy,biofilms,scanning electron microscopy in back-scattered mode,lithobionts,biodeterioro,microscopia in situ,biofilmes,microscopia eletrônica de varredura em modo back-scattered,lithobiontes,biopelículas,microscopia electrónica de barrido en modo back-scattered,litobiontes

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