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      Taste sensitivity to a mixture of monosodium glutamate and inosine 5′-monophosphate by mice lacking both subunits of the T1R1+T1R3 amino acid receptor

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          Abstract

          The taste of l-glutamate and its synergism with 5′-ribonucleotides is thought to be primarily mediated through the T1R1+T1R3 heterodimer in some mammals, including rodents and humans. While knockout (KO) mice lacking either receptor subunit show impaired sensitivity to a range of monosodium glutamate (MSG) concentrations mixed with 2.5 mM inosine 5′-monophosphate (IMP) in amiloride, wild-type (WT) controls can detect this IMP concentration, hindering direct comparison between genotypes. Moreover, some residual sensitivity persists in the KO group, suggesting that the remaining subunit could maintain a limited degree of function. Here, C57BL/6J, 129X1/SvJ, and T1R1+T1R3 double KO mice ( n = 16 each to start the experiment) were trained in a two-response operant task in gustometers and then tested for their ability to discriminate 100 µM amiloride from MSG (starting with 0.6 M) and IMP (starting with 2.5 mM) in amiloride (MSG+I+A). Testing continued with successive dilutions of both MSG and IMP (in amiloride). The two WT strains were similarly sensitive to MSG+I+A ( P > 0.8). KO mice, however, were significantly impaired relative to either WT strain ( P < 0.01), although they were able to detect the highest concentrations. Thus, normal detectability of MSG+I+A requires an intact T1R1+T1R3 receptor, without regard for allelic variation in the T1R3 gene between the WT strains. Nevertheless, residual sensitivity by the T1R1+T1R3 KO mice demonstrates that a T1R-independent mechanism can contribute to the detectability of high concentrations of this prototypical umami compound stimulus.

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          Author and article information

          Journal
          Am J Physiol Regul Integr Comp Physiol
          Am. J. Physiol. Regul. Integr. Comp. Physiol
          ajpregu
          Am J Physiol Regul Integr Comp Physiol
          AJPREGU
          American Journal of Physiology - Regulatory, Integrative and Comparative Physiology
          American Physiological Society (Bethesda, MD )
          0363-6119
          1522-1490
          1 June 2018
          14 February 2018
          1 June 2019
          : 314
          : 6
          : R802-R810
          Affiliations
          1Department of Psychology and Program in Neuroscience, Florida State University , Tallahassee, Florida
          2Division of Biosciences, College of Dentistry, Ohio State University , Columbus, Ohio
          Author notes
          Address for reprint requests and other correspondence: A. C. Spector, Dept. of Psychology and Program in Neuroscience, Florida State Univ., 1107 W. Call St., Tallahassee, FL, 32306 (e-mail: spector@ 123456psy.fsu.edu ).
          Article
          PMC6032298 PMC6032298 6032298 R-00352-2017 R-00352-2017
          10.1152/ajpregu.00352.2017
          6032298
          29443544
          Copyright © 2018 the American Physiological Society
          Funding
          Funded by: HHS | NIH | National Institute on Deafness and Other Communication Disorders (NIDCD) 10.13039/100000055
          Award ID: R01-DC004574
          Categories
          Research Article
          Neural Control

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