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      Do drowning and anoxia kill head lice? Translated title: La noyade et l’anoxie tuent-elles les poux de tête ?

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          Abstract

          Chemical, physical, and mechanical methods are used to control human lice. Attempts have been made to eradicate head lice Pediculus humanus capitis by hot air, soaking in various fluids or asphyxiation using occlusive treatments. In this study, we assessed the maximum time that head lice can survive anoxia (oxygen deprivation) and their ability to survive prolonged water immersion. We also observed the ingress of fluids across louse tracheae and spiracle characteristics contrasting with those described in the literature. We showed that 100% of lice can withstand 8 h of anoxia and 12.2% survived 14 h of anoxia; survival was 48.9% in the untreated control group at 14 h. However, all lice had died following 16 h of anoxia. In contrast, the survival rate of water-immersed lice was significantly higher when compared with non-immersed lice after 6 h (100% vs. 76.6%, p = 0.0037), and 24 h (50.9% vs. 15.9%, p = 0.0003). Although water-immersed lice did not close their spiracles, water did not penetrate into the respiratory system. In contrast, immersion in colored dimeticone/cyclomethicone or colored ethanol resulted in penetration through the spiracles and spreading to the entire respiratory system within 30 min, leading to death in 100% of the lice.

          Translated abstract

          Des méthodes chimiques, physiques et mécaniques sont utilisées pour contrôler les poux humains. Des tentatives ont été faites pour éradiquer les poux de tête Pediculus humanus capitis par l’air chaud, en les trempant dans divers fluides ou en les asphyxiant à l’aide de traitements occlusifs. Dans cette étude, nous avons évalué le temps maximum que les poux de tête peuvent survivre à l’anoxie (privation d’oxygène) et leur capacité à survivre à une immersion prolongée dans l’eau. Nous avons également observé la pénétration de fluides à travers les trachées des poux et les caractéristiques du spiracle, contrastant avec celles décrites dans la littérature. Nous avons montré que 100 % des poux supportent 8 h d’anoxie et que 12,2 % survivent à 14 h d’anoxie ; 48,9 % de survie à 14h a été obtenu dans le groupe témoin non traité. Cependant, tous les poux sont morts après 16 h d’anoxie. En revanche, le taux de survie des poux immergés était significativement plus élevé que celui des poux non immergés après 6 h (100 % contre 76,6 %, p = 0,0037) et 24 h (50,9 % contre 15,9 %, p = 0,0003). Bien que les poux immergés dans l’eau n’aient pas fermé leurs spiracles, l’eau n’a pas pénétré dans le système respiratoire. En revanche, l’immersion dans de la diméticone/cyclométhicone colorée ou de l’éthanol coloré a entraîné une pénétration à travers les spiracles et une propagation à l’ensemble du système respiratoire dans les 30 minutes, entraînant la mort de 100 % des poux.

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          Most cited references 45

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          Scabies and pediculosis.

           O Chosidow (2000)
          Scabies and pediculosis are ubiquitous, contagious, and debilitating parasitic dermatoses. They have been known since antiquity and are distributed worldwide. Clusters of infestation occur-for example, scabies affecting immunocompromised individuals or patients and staff in hospitals and nursing homes for the elderly, and pediculosis affecting schoolchildren or homeless people. Associations with other disorders are common: infections with human T-cell leukaemia/lymphoma virus I (HTLV-I) and HIV are associated with scabies, and trench fever and exanthematous typhus with pediculosis. Specific forms of scabies, including bullous scabies or localised crusted scabies, may be misdiagnosed. Moreover, definitive parasitic diagnosis can be difficult to obtain, and the value of new techniques remains to be confirmed. Difficulties in management have returned scabies and pediculosis to the limelight.
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            Insecticide resistance in head lice: clinical, parasitological and genetic aspects.

            Insecticide treatment resistance is considered to be a major factor in the increasing number of infestations by head lice. The large insecticide selection pressure induced by conventional topical pediculicides has led to the emergence and spread of resistance in many parts of the world. Possible mechanisms of resistance include accelerated detoxification of insecticides by enzyme-mediated reduction, esterification, oxidation that may be overcome by synergistic agents such as piperonyl butoxide, alteration of the binding site, e.g. altered acetylcholinesterase or altered nerve voltage-gated sodium channel, and knockdown resistance (kdr). Clinical, parasitological and molecular data on resistance to conventional topical pediculicides show that treatments with neurotoxic insecticides have suffered considerable loss of activity worldwide. In particular, resistance to synthetic pyrethroids has become prominent, probably because of their extensive use. As other treatment options, including non-insecticidal pediculicides such as dimeticone, are now available, the use of older insecticides, such as lindane and carbaryl, should be minimized, owing to their loss of efficacy and safety concerns. The organophosphorus insecticide malathion remains effective, except in the UK, mostly in formulations that include terpineol. © 2012 The Authors. Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases.
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              Bartonella quintana in Body Lice and Head Lice from Homeless Persons, San Francisco, California, USA

              The human body louse and human head louse are generally recognized as 2 subspecies of Pediculus humanus (P. h. humanus and P. h. capitis, respectively) that have distinct ecologic preferences ( 1 ). However, a recent genetic analysis was not able to show any differences between these 2 subspecies ( 2 ). The human body louse is a small, parasitic insect that lives on the body and in the clothing or bedding of its human host. Body lice feed only on human blood. In the United States, body lice infestations usually are found only on persons who do not have access to clean changes of clothes or bathing facilities (e.g., the homeless population). Head lice also feed only on human blood and are found on the head. Head lice infestations occur most often in school children and may also occur in the homeless population where they may be transferred by pillow cases, hats, and combs. Body and head lice are morphologically indistinguishable by the unaided human eye. Body lice are most reliably differentiated from head lice by their presence on clothing or on parts of the body other than the head or neck. These lice spend most of their time on the clothing of an infested person, visiting the body up to 5 times a day to feed. The eggs (called nits) of body lice are cemented to clothing fibers and seams or, occasionally, to body hairs ( 3 , 4 ). In addition to causing discomfort and irritation, body lice can transmit disease-causing pathogens. Bartonella quintana is a bacterium transmitted through body lice feces that are scratched into the skin by the host. This organism can cause trench fever, endocarditis, bacillary angiomatosis, peliosis, and chronic bacteremia in infected humans ( 3 ). Since 1992, B. quintana has been recognized as a reemerging infection in homeless populations in the United States and Europe, as well as an opportunistic pathogen in patients with AIDS ( 5 ). Infection with B. quintana can cause prolonged disability in immunocompetent persons and can be life-threatening in immunodeficient patients. Studies of homeless persons seeking medical care in clinics and hospitals in the United States and France have found that 2%–20% of persons tested had antibodies against B. quintana ( 6 – 9 ). In Tokyo, Japan, 57% of homeless patients had immunoglobulin (Ig) G titers >128 for B. quintana ( 10 ). A study in Marseille, France, found that 14% (10/71) of homeless patients who came to a hospital emergency department had blood cultures positive for B. quintana ( 11 ). In 1990, physicians in the San Francisco, California, Bay area recognized the link between Bartonella spp. infections and bacillary angiomatosis ( 12 , 13 ) and bacillary peliosis hepatis ( 14 ). A subsequent study by Koehler et al. documented the occurrence of bacillary angiomatosis in 49 patients seen over 8 years ( 15 ). All patients in this study were infected with either B. quintana or B. henselae (the agent of cat-scratch disease), most case-patients were immunocompromised (92% had HIV infection), and B. quintana infection was associated with homelessness and body lice infestation. In a subsequent study of HIV-positive patients with fever in San Francisco, Koehler and others found that 18% of 382 patients were positive for Bartonella spp. ( 16 ). The human body louse is currently thought to play a role in the transmission of B. quintana among homeless persons, much as it did during the epidemics of trench fever that occurred during World Wars I and II ( 3 ). In the aforementioned study in Marseille, France, in 1999, body lice from 3 (20%) of 15 homeless patients were positive for B. quintana by PCR ( 11 ). In Tokyo, Sasaki et al. examined clothing from 12 homeless persons for body lice ( 17 ). These authors found that lice from 2 (16.7%) of 12 homeless persons were positive for B. quintana by PCR ( 17 ). Furthermore, evidence now indicates that head lice may be involved in the transmission cycle of B. quintana ( 18 ). Homeless populations in urban areas in northern California are vulnerable to body lice infestation and may be at risk for B. quintana infection. We studied whether body and head lice from homeless populations in a northern California city are carrying B. quintana or other pathogenic Bartonella spp. Materials and Methods In 2007 and 2008, staff from the Vector-borne Diseases Section of the California Department of Public Health (CDPH) participated in San Francisco’s Project Homeless Connect (SFPHC). Under the auspices of SFPHC medical services, hair, body, and clothing of homeless persons were inspected for lice. Any lice found on the head with the presence of nits were considered to be head lice. Any lice on the body or clothing were considered to be body lice. Most infested persons self-referred directly to the CDPH booth at this event, with the exception of 1 physician referral. Lice were collected by using forceps, identified, sorted by subspecies, and placed in screw-top vials filled with 95% ethanol. Only a portion of the total lice infesting a person were collected for testing. The lice were shipped to the Centers for Disease Control and Prevention (Fort Collins, CO, USA) for detection and identification of Bartonella spp. Lice were pooled by host and then subspecies. Samples from hosts with >20 lice were further tested individually to obtain an estimate of Bartonella spp. prevalence in the lice. We tested 36 pools of body lice, 7 pools of head lice, 108 individual body lice, and 4 individual head lice. Individual or pooled (2–20 lice/pool) samples were suspended in 250 μL of sterile phosphate-buffered saline and homogenized in an MM300 mixer (Retsch, Newtown, PA, USA) for 8 min. DNA was extracted from the homogenates by using a Mini Kit (QIAGEN, Valencia, CA, USA) and the Blood and Body Fluid Spin Protocol according to the manufacturer’s protocol with a few minor changes. A PCR was performed in 20 μL of the mixtures containing 4–20 ng of the extracted DNA, 20 μL of 2× Ampdirect Plus, 0.5 U of Ex Taq Hot Start Version (Takara Bio, Otsu, Japan), and 1 pmol of each primer. Bartonella DNA was amplified by using gltA (citrate synthase gene) and ftsZ (cell division protein gene) primers as reported ( 19 , 20 ) in a thermalcycler (iCycler; BioRad, Hercules, CA, USA). A strain of B. washoensis was used as a positive control, and sterile deionized water was used as a negative control. Using gel electrophoresis on a 2% agarose gel, we examined the PCR products for 900-bp (ftsZ) and 380–400-bp (gltA) fragments. The PCR amplicon of each gene was purified by using a QIA quick PCR purification kit (QIAGEN). Direct DNA sequencing of the purified PCR amplicons was conducted by using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) with the specific primers described above on a Model 3130X Genetic Analyzer (Applied Biosystems). Sequence data of each gene were aligned and compared with type strains of Bartonella spp. in GenBank by using MegAlign software (DNA Star Inc., Madison, WI, USA). Results In 2007 and 2008, 138 homeless persons had consultations at the CDPH booth at the SFPHC event. Of these persons, CDPH staff observed 33 persons with body lice infestations (23.9%) and 624 lice were collected (mean 18.9 lice/infested host). Head lice infestations were detected in 12 (8.7%) persons and 70 lice were collected (mean 5.8 lice/infested host). Six persons (4.3%) had body lice and head lice infestations. Bartonella DNA was detected in body lice collected from 11 (33.3%) persons (Table 1) and in head lice collected from 3 (25.0%) persons (Table 2). Nine pools of body lice (n = 2–20, mean infection rate [MIR] 5%) from 9 infested persons and 2 pools of head lice (n = 7–12, MIR 8.3%) from 1 infested person showed evidence of Bartonella DNA. Additional lice from persons with positive pooled samples of body lice (SFB6 and SFB24) were tested individually. Sample SFB6 had 13 (87%) of 15 lice positive for Bartonella DNA. Sample SFB24 had 27 (64%) of 42 lice positive for Bartonella DNA. One of the 4 individual head louse samples (SFH2) showed amplification of Bartonella DNA (Table 2). Table 1 Bartonella quintana in body lice (1–20 lice/ sample) from homeless persons, San Francisco, California, USA Specimen ID Date collected No. samples tested (no. lice) No. positive samples* gltA ftsZ SFB1 2007 Feb 1 (2) 0 0 SFB2 2007 Feb 1 (1) 0 0 SFB3 2007 Feb 1 (1) 0 0 SFB4 2007 Feb 1 (4) 0 0 SFB5 2007 Feb 1 (1) 0 0 SFB6 2007 Apr 16 (35) 14 13 SFB7 2007 Apr 1 (2) 0 0 SFB8 2007 Apr 1 (4) 0 0 SFB9 2007 Aug 3 (22) 0 0 SFB10 2007 Aug 4 (23) 0 0 SFB11 2007 Aug 1 (7) 0 0 SFB12 2007 Aug 1 (14) 0 0 SFB13 2007 Aug 1 (2) 1 1 SFB14 2007 Dec 1 (2) 0 0 SFB15 2007 Dec 1 (4) 0 0 SFB16 2007 Dec 5 (91) 1 0 SFB17 2007 Dec 2 (40) 1 1 SFB18 2007 Dec 1 (6) 1 0 SFB19 2007 Dec 1 (1) 1 0 SFB20 2007 Dec 1 (5) 0 0 SFB21 2008 Jan 1 (16) 1 0 SFB22 2008 Jan 2 (21) 1 0 SFB23 2008 Jan 1 (2) 0 0 SFB24 2008 Jan 43 (62) 26 24 SFB25 2008 Jan 6 (25) 0 0 SFB26 2008 Jan 1 (3) 1 0 SFB27 2008 Jan 8 (27) 1 0 SFB72 2008 Jun 6 (25) 0 0 SFB73 2008 Jun 7 (7) 0 0 SFB74 2008 Jun 2 (2) 0 0 SFB76 2008 Jun 9 (85) 0 0 SFB77 2008 Jun 10 (10) 0 0 SFB78 2008 Jun 12 (12) 0 0 *gltA, citrate synthase gene; ftsZ, cell division protein gene. Table 2 Bartonella quintana in head lice (1–20 lice/sample) from homeless persons, San Francisco, California, USA Specimen ID Date collected No samples tested (no. lice) No. positive samples* gltA ftsZ SFH1 2007 Feb 1 (7) 1 0 SFH2 2007 Apr 1 (1) 1 1 SFH3 2007 Aug 2 (32) 1 0 SFH4 2007 Dec 1 (15) 0 0 SFH5 2007 Dec 1 (2) 0 0 SFH6 2007 Dec 1 (2) 0 0 SFH7 2007 Dec 1 (4) 0 0 SFH8 2008 Jan 1 (1) 0 0 SFH75 2008 Jun 2 (2) 0 0 SFH79 2008 Jun 1 (1) 0 0 SFH80 2008 Jun 2 (2) 0 0 SFH81 2008 Jun 1 (1) 0 0 *gltA, citrate synthase gene; ftsZ, cell division protein gene. Host sample SFB16 showed no amplification of Bartonella DNA in its original test but when an additional 3 pools of 20 lice and 11 individual lice were tested, 1 pool of 20 lice was positive. This host had a massive body louse infestation; 91 lice were collected from his clothing. Host sample SFB27 was also negative in its first test of a pool of 20 lice; 7 additional lice tested afterwards produced a single detection of B. quintana DNA in a body louse (14%). Samples from 1 person who was co-infested with body lice and head lice were positive for Bartonella DNA by the gltA gene PCR (SFB17, 1 pool of 20 lice) in body lice, but not in the head lice pool (SFH7, n = 4). Samples from another co-infested person were negative for Bartonella DNA in 1 pool of 5 body lice (SFB10). Bartonella DNA was detected in a pool of 12 head lice (SFH3, MIR 8.3%) (Tables 1, 2). Thirteen (86.7%) and 12 (80.0%) body lice from host SFB6 had Bartonella DNA amplification by gltA and ftsZ, respectively. Twenty-five (59.5%) and 23 (54.8%) of individual body lice from host SFB24 had Bartonella DNA amplification by gltA and ftsZ, respectively. Two samples from hosts SFB6 and SFB24 were sequenced and found to be identical with B. quintana type strain Fuller for both genes. Host sample SFB13 had Bartonella DNA amplification for both genes, and showed a sequence identical to B. quintana type strain Fuller for the gltA product and 99.9% homology to the same type strain for the ftsZ product. One of the individual head lice samples, SFH2, showed positive amplification of the ftsZ and gltA genes. Sequencing showed that this sample was B. quintana type strain Fuller for the gltA product. Discussion Our study has shown that homeless persons in the San Francisco Bay area have body and head lice that harbor B. quintana type strain Fuller. Prevalence of B. quintana in body lice from homeless persons (33.3%) in our study was slightly higher than the prevalence reported by Sasaki et al. in Tokyo, where body lice in 2 (16.7%) of 12 homeless persons were infected with B. quintana ( 17 ). Furthermore, similar prevalence of B. quintana infection in body lice has been reported from Russia (12.3%) ( 21 ) and Marseille, France (20%) ( 11 ). Although Sasaki et al. detected B. quintana DNA in head lice by using molecular detection methods, their samples came from children in Nepal who also had body lice ( 18 ). However, there is no strong evidence that head lice are vectors of this organism between human hosts. Moreover, Fournier and others tested 143 head lice from schoolchildren from 8 countries and found no B. quintana ( 22 ) We have detected B. quintana in head lice from persons without a known concurrent body louse infestation. Further work is needed to examine how homeless persons acquire lice and which groups may be predisposed to louse infestation and B. quintana infection.
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                Author and article information

                Journal
                Parasite
                Parasite
                parasite
                Parasite
                EDP Sciences
                1252-607X
                1776-1042
                2018
                9 March 2018
                : 25
                : ( publisher-idID: parasite/2018/01 )
                Affiliations
                [1 ] Parasitology-Mycology Department, Avicenne Hospital, AP-HP, Bobigny France
                [2 ] Unit of Pharmacology, Avicenne Hospital, AP-HP, Bobigny France
                [3 ] Unité des Virus Emergents (Aix-Marseille Univ – IRD 190 – Inserm 1207 – IHU Méditerranée infection), Marseille France
                Author notes
                [* ]Corresponding author: Laboratoire de Parasitologie-Mycologie, Hôpital Avicenne, 125 rue de Stalingrad, 93009 Bobigny Cedex, France. candykerdalidec@ 123456yahoo.fr
                Article
                parasite170108 10.1051/parasite/2018015
                10.1051/parasite/2018015
                5844234
                29521258
                © K. Candy et al., published by EDP Sciences, 2018

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                Page count
                Figures: 4, Tables: 0, Equations: 0, References: 57, Pages: 9
                Categories
                Research Article

                pediculus humanus capitis, drowning, water, anoxia, oxygen

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