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      Simultaneous determination of lithospermic acid B and its three metabolites by liquid chromatography/tandem mass spectrometry.

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          Abstract

          A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed for the simultaneous determination of lithospermic acid B and its three main O-methylated metabolites in rat serum with silibinin as the internal standard. The calibration curves for LSB, and the three metabolites were linear over the ranges of 16-4096, and 8-2048 ng/ml, respectively, with coefficients of correlation >0.998. For LSB, the intra-assay coefficient of variance (CV) was less than 9.3% and the inter-assay CV was less than 8.9%. The inter-assay mean accuracy was between 92.8% and 104.7%. For the three metabolites, the intra-assay CV was less than 8.7% and the inter-assay CV was less than 9.9%. The inter-assay mean accuracy was between 92.5% and 107.9%. This quantitation method was successfully applied to a pharmacokinetic study of LSB in rats. Also, a total recovery of 5.2% was found in bile after oral administration.

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          Author and article information

          Journal
          J Pharm Biomed Anal
          Journal of pharmaceutical and biomedical analysis
          Elsevier BV
          0731-7085
          0731-7085
          Apr 11 2007
          : 43
          : 5
          Affiliations
          [1 ] State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
          Article
          S0731-7085(07)00020-9
          10.1016/j.jpba.2007.01.004
          17275241

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