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      Transgenic mouse line with green-fluorescent protein-labeled Centrin 2 allows visualization of the centrosome in living cells.

      Transgenic Research
      Animals, Calcium-Binding Proteins, analysis, genetics, metabolism, Cell Nucleus, ultrastructure, Centrioles, Centrosome, Embryo, Mammalian, cytology, Fibroblasts, Green Fluorescent Proteins, Mice, Mice, Transgenic, Microscopy, Fluorescence, Recombinant Fusion Proteins

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          Abstract

          The centrosome plays diverse roles throughout the cellular mitotic cycle and in post-mitotic cells. Analysis of centrosome position and dynamics in living murine cells has been limited due to a lack of adequate reporters and currently requires either cell fixation/immunostaining or transfection with centrosome reporters. Here we describe the generation and characterization of a transgenic mouse line that constitutively expresses green fluorescent protein-labeled Centrin-2 (GFP-CETN2). The phenotype of the mouse is indistinguishable from wild-type and it displays a single pair of fluorescent centrioles in cells of every organ and time point examined. This model will be helpful for visualizing the centrosome in multiple experimental conditions.

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