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      Advances in fluorescent protein technology.

      Journal of Cell Science

      methods, Protein Engineering, Protein Conformation, Microscopy, Fluorescence, chemistry, Luminescent Proteins, Green Fluorescent Proteins, Fluorescence Resonance Energy Transfer, Fluorescence Recovery After Photobleaching, Animals

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          Abstract

          Current fluorescent protein (FP) development strategies are focused on fine-tuning the photophysical properties of blue to yellow variants derived from the Aequorea victoria jellyfish green fluorescent protein (GFP) and on the development of monomeric FPs from other organisms that emit in the yellow-orange to far-red regions of the visible light spectrum. Progress toward these goals has been substantial, and near-infrared emitting FPs may loom over the horizon. The latest efforts in jellyfish variants have resulted in new and improved monomeric BFP, CFP, GFP and YFP variants, and the relentless search for a bright, monomeric and fast-maturing red FP has yielded a host of excellent candidates, although none is yet optimal for all applications. Meanwhile, photoactivatable FPs are emerging as a powerful class of probes for intracellular dynamics and, unexpectedly, as useful tools for the development of superresolution microscopy applications.

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          Author and article information

          Journal
          10.1242/jcs.005801
          18057027

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