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Abstract
Xylan and rice straw were used to produce H(2) via a two-stage approach combining
feedstock pretreatment/hydrolysis and dark H(2) fermentation. Acinetobacter junii
F6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan
and pretreated rice straw. The hydrolysates were converted to H(2) by Clostridium
butyricum CGS5 via dark fermentation. Investigation of kinetics of xylanase on xylan
and NaOH-pretreated rice straw shows nu(max) values of 8.6 and 3.6g/L/h, and K(m)
values of 10.6 and 26.9 g/L, respectively. A maximum hydrogen production rate of 62.5
and 26.8 ml/h/L was obtained from hydrolysate of xylan and pretreated rice straw,
respectively, while the hydrogen yield was 0.70 and 0.76 mol H(2)/mol xylose, respectively.
Simultaneous saccharification and BioH(2) fermentation from xylan was also conducted
but giving a lower hydrogen production rate (35.3 ml/h/L) than that of the two-stage
process.
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