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Molecular characterization and overexpression of mnp6 and vp3 from Pleurotus ostreatus revealed their involvement in biodegradation of cotton stalk lignin

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      Fungal secretory heme peroxidase (Class II POD) plays a significant role in biomass conversion due to its lignin-degrading activity. In this study, genome-wide identification and bioinformatics were performed to analyze Pleurotus ostreatus peroxidases (PoPODs). A total of six manganese peroxidases (MnPs) and three versatile peroxidases (VPs) were obtained. Bioinformatics analysis and qRT-PCR showed that P. ostreatus mnp6 (Pomnp6) and P. ostreatus vp3 (Povp3) could be involved in lignin degradation. Both Pomnp6 and Povp3 transgenetic fungi showed significantly increased lignin degradation of cotton stalks. 1H-NMR revealed that Pomnp6 and Povp3 may preferentially degrade S-lignin in cotton stalks and mainly break β-O-4′ bond linkages and hydroxyl. These results support the possible utility of Pomnp6 and Povp3 in natural straw resources and development of sustainable energy.


      Summary: Pleurotus ostreatus mnp6 and vp3 could degrade cotton stalk lignin and may preferentially degrade S-lignin.

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            Author and article information

            [1 ]School of Life Sciences, Anhui Agricultural University , Hefei 230036, China
            [2 ]Horticultural Research Institute, Anhui Academy of Agricultural Sciences , Hefei 230031, China
            Author notes

            These authors contributed equally to this work.

            []Author for correspondence ( swkx12@ ; fan.n@ )
            Biol Open
            Biol Open
            Biology Open
            The Company of Biologists Ltd
            15 February 2019
            24 December 2018
            24 December 2018
            : 8
            : 2
            © 2019. Published by The Company of Biologists Ltd

            This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

            Funded by: Anhui Academy of Agricultural Sciences Youth Innovation Fund Project;
            Award ID: 14B0322
            Funded by: Technical System of Anhui Vegetable Industry;
            Award ID: AHCYJSTX-09-05
            Research Article


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