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Six, one-week-old gnotobiotic piglets were inoculated with tissues or sera collected
from field cases of porcine reproductive and respiratory syndrome. The piglets showed
little or no illness, and two that were necropsied at 8 and 9 days post infection
appeared grossly normal. However, a Lelystad virus-like agent was isolated from most
of the inoculated pigs using porcine alveolar macrophage cultures. Seroconversion
to the Lelystad virus was observed and some animals developed microscopically detectable
interstitial pneumonias. Scanning electron microscopy was used to study the in vitro
cytopathic effect of the Lelystad virus on porcine alveolar macrophages.