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      Isolation of a Lelystad virus-like agent from British pigs and scanning electron microscopy of infected macrophages

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      Veterinary Microbiology

      Elsevier BV

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          Abstract

          Six, one-week-old gnotobiotic piglets were inoculated with tissues or sera collected from field cases of porcine reproductive and respiratory syndrome. The piglets showed little or no illness, and two that were necropsied at 8 and 9 days post infection appeared grossly normal. However, a Lelystad virus-like agent was isolated from most of the inoculated pigs using porcine alveolar macrophage cultures. Seroconversion to the Lelystad virus was observed and some animals developed microscopically detectable interstitial pneumonias. Scanning electron microscopy was used to study the in vitro cytopathic effect of the Lelystad virus on porcine alveolar macrophages.

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          Author and article information

          Journal
          Veterinary Microbiology
          Veterinary Microbiology
          Elsevier BV
          03781135
          November 1992
          November 1992
          : 33
          : 1-4
          : 195-201
          Article
          1481357
          © 1992

          https://www.elsevier.com/tdm/userlicense/1.0/

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