Rapid Detection of Cytomegalovirus DNA in Sera Using the Polymerase Chain Reaction: Relationship with Clinical Diagnosis of Cytomegalovirus Infection after Renal Transplantation

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Abstract

A 21-year-old man had chronic renal failure and received a renal transplant. We used the polymerase chain reaction technique to detect cytomegalovirus (CMV) DNA in serum, blood, and urine for the diagnosis of CMV infection after the renal transplantation. CMV DNA in the serum was detected by polymerase chain reaction only during the active stage, while CMV DNA in urine and blood was detected even during the silent stage of CMV infection. It is suggested that the use of the polymerase chain reaction in serum would be useful for the rapid diagnosis of active CMV infection.

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Author and article information

Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 1995

Publication date (Print): 1995

Publication date (Electronic): 18 December 2008

Volume: 70

Issue: 1

Pages: 100-103

Affiliations

Departments of ^aClinical Pathology and ^bInternal Medicine, Showa University Fujigaoka Hospital, Yokohama; ^cDepartment of Cell Regulation, Division of Virology and Immunology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan

Article

Publisher ID: 188551 Other ID: Nephron 1995;70:100–103

DOI: 10.1159/000188551

PubMed ID: 7617086

SO-VID: 473313ff-bf6f-499b-bb32-e4920f210914

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date accepted : 31 March 1994

Page count

Pages: 4

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