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      Actin-ATP Hydrolysis Is a Major Energy Drain for Neurons

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          Abstract

          In cultured chick ciliary neurons, when ATP synthesis is inhibited, ATP depletion is reduced ∼50% by slowing actin filament turnover with jasplakinolide or latrunculin A. Jasplakinolide inhibits actin disassembly, and latrunculin A prevents actin assembly by sequestering actin monomers. Cytochalasin D, which allows assembly–disassembly, but only at pointed ends, is less effective in conserving ATP. Ouabain, an Na +–K +-ATPase inhibitor, and jasplakinolide both prevent ∼50% of the ATP loss. When applied together, they completely prevent ATP loss over a period of 20 min, suggesting that filament stabilization reduces ATP consumption by decreasing actin-ATP hydrolysis directly rather than indirectly by modulating the activity of Na +–K +-ATPase, a major energy consumer.

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          Author and article information

          Journal
          J Neurosci
          J. Neurosci
          jneuro
          jneurosci
          J. Neurosci
          The Journal of Neuroscience
          Society for Neuroscience
          0270-6474
          1529-2401
          1 January 2003
          : 23
          : 1
          : 1-6
          Affiliations
          [ 1 ]Department of Biochemistry and Molecular Biology and the Program in Molecular, Cellular, and Integrative Neuroscience, Colorado State University, Fort Collins, Colorado 80523-1870
          Article
          PMC6742122 PMC6742122 6742122 7160
          10.1523/JNEUROSCI.23-01-00002.2003
          6742122
          12514193
          4741ce6d-aa31-4854-8243-3a7c77f26eec
          Copyright © 2003 Society for Neuroscience
          History
          : 14 June 2002
          : 13 September 2002
          : 16 October 2002
          Categories
          Brief Communication
          Custom metadata
          5.00
          1

          latrunculin , ischemia , cytoskeleton , actin filament treadmilling , intracellular ATP , jasplakinolide

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