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      c-Fos Expression in the Rat Cerebral Cortex during Systemic GvH Reaction

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          Abstract

          Objective: It is becoming clear that the CNS receives signals from the peripheral immune system. In order to identify the areas of the brain that receive information about a specific immune response to allogeneic antigens, we studied the expression of c-Fos, a neural activation marker, in the cerebral cortex following the induction of a graft-vs.-host reaction (GvHR) in rats. Methods: C-Fos expression in the brain was studied by immunohistochemistry. GvHR was induced in (WKY × PVG)F<sub>1</sub> rats by injecting 5 × 10<sup>8</sup> spleen cells from PVG rats. Control rats received syngeneic cells. Results: No c-Fos immunoreactivity (IR) was observed in animals undergoing GvHR in the nucleus tractus solitarii (NTS), the locus coeruleus (LC), the organum vasculosum of lamina terminalis (OVLT), the paraventricular nucleus (PVN) or the central amygdaloid nucleus (Ce). In contrast, 3 days after GvH induction c-Fos IR was observed in the piriform cortex and several other olfactory-related regions indicating the stimulation of the olfactory pathway during GvHR. Strong c-Fos IR was also observed in the occipital visual cortex of animals undergoing a GvHR, suggesting that GvHR can affect visual functions. In addition, GvHR induced c-Fos IR in the prefrontal cortex (Cg3, orbital cortex), a region that has interconnections with most sensory modalities. Double-staining studies indicate that the cells that express the c-Fos signal are neurons. Conclusion: We have defined the distribution of brain neurons that are affected during the induction phase of GvHR. Our results also indicate that the integration and processing of information from the immune system at CNS levels involve different areas during different types of immune responses.

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          Odor-induced fos-like immunoreactivity in the rat olfactory bulb.

          We here report odor-induced mapping patterns of c-fos-like protein (Fos) immunoreactivity in the rat olfactory bulb under urethane anesthesia. Regional patterns of cells with nuclei expressing Fos, plotted on a spread-out reconstruction of the mitral cell layer, were strikingly comparable to data from the 2-deoxyglucose method, by which different foci of the most-labelled glomeruli of metabolic activity were demonstrated using different odors. Cells in the glomerular, external plexiform, and granule cell layers were also labelled.
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            Author and article information

            Journal
            NIM
            Neuroimmunomodulation
            10.1159/issn.1021-7401
            Neuroimmunomodulation
            S. Karger AG
            1021-7401
            1423-0216
            2004
            October 2004
            13 October 2004
            : 11
            : 6
            : 425-433
            Affiliations
            aSecond Department of Anatomy, Hamamatsu University, School of Medicine, Hamamatsu, Japan; bDepartment Immunophysiology, Medical Faculty, Philipps University, Marburg, Germany
            Article
            80154 Neuroimmunomodulation 2004;11:425–4330
            10.1159/000080154
            15467359
            © 2004 S. Karger AG, Basel

            Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

            Page count
            Figures: 9, References: 43, Pages: 9
            Categories
            Original Paper

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