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      Polyphenols isolated from Acacia mearnsii bark with anti-inflammatory and carbolytic enzyme inhibitory activities

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          Abstract

          The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g −1, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g −1, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme (IC 50 of 0.4–1.4 μg·mL −1) than the pharmaceutical acarbose (IC 50 141.8 μg·mL −1). B6 and B7 (IC 50 17.6 and 11.7 μg·mL −1, respectively) exhibited α-amylase inhibitory activity as efficacious as acarbose (IC 50 15.4 μg·mL −1). Moreover, B extract, at 25 μg·mL −1, significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.

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          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 November 2017
          : 15
          : 11
          : 816-824
          Affiliations
          1Department of Chemical Engineering, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China
          2Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA
          Author notes
          *Corresponding author: LILA Mary Ann, Tel: +1 704.250.5407, Fax: +1 704.250.5409, E-mail: mlila@ 123456ncsu.edu

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(18)30015-3
          10.1016/S1875-5364(18)30015-3
          Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and State Forestry Administration of China
          Award ID: 201104019
          This work was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and State Forestry Administration of China (No. 201104019).

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