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      Evaluation of Petrifilm™ system compared with traditional methodology in count of indicators of sanitary-hygienic quality and pathogenic microorganisms in sheep milk

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          Abstract

          The objective of this study was to evaluate the applicability of the Petrifilm™ plates to enumerate microbial groups in sheep milk. Samples of sheep milk (n = 30) were plated simultaneously, to enumerate mesophilic aerobes, total coliforms, lactic acid bacteria, Staphylococcus aureus and Escherichia coli, using convencional reference protocols and Petrifilm™ plates. The results were compared using McNemar's test, linear regression and ANOVA (p < 0,05). The results demonstrated good significant between conventional methodologies and Petrifilm™ plates. Further, the Petrifim™ STX for counting S. aureus had higher recoverability of bacteria compared with the conventional methodology. Based on the results obtained and in view of the ease and rapidity procedures results, Petrifim ™ plates may be considered as alternatives for microbiological testing in sheep milk.

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          Most cited references16

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          Standard Methods for the Examination of Dairy Products

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            Enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese: An evaluation of Baird-Parker agar, Rabbit Plasma Fibrinogen agar and the Petrifilm™ Staph Express count system

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              Comparison of Petrifilm aerobic count plates and de Man-Rogosa-Sharpe agar for enumeration of lactic acid bacteria

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                cta
                Food Science and Technology (Campinas)
                Food Sci. Technol (Campinas)
                Sociedade Brasileira de Ciência e Tecnologia de Alimentos (Campinas )
                1678-457X
                June 2015
                : 35
                : 2
                : 375-379
                Affiliations
                [1 ] Universidade de Brasília Brazil
                [2 ] Universidade Federal de Viçosa Brazil
                Article
                S0101-20612015000200375
                10.1590/1678-457X.6430
                4848f098-703d-4498-94a1-1b53e468ceba

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0101-2061&lng=en
                Categories
                FOOD SCIENCE & TECHNOLOGY

                Food science & Technology
                milk analyzes,sheep's milk,Escherichia coli,Aerobic mesophilic bacteria

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