Phytochemical constituents and biological activities of Cleome iberica DC.

Abstract

Phytochemical investigation of Cleome iberica DC. aerial parts led to the isolation and characterisation of six triterpenoid including glutinol (1), lupeol (2), β-sitosterol (3), cabraleadiol monoacetate (4), cabraleadiol (6) and daucosterol (8), one flavonoid, calycopterin (5) along with a fatty acid, tricosanoic acid (7). While studied samples showed weak potency in antimicrobial assessment, the acetone extract and some isolated compounds exhibited considerable cytotoxicity against cervical cancer cell line (HeLa) in MTT assay, among them calycopterin with IC50 equals to 5.1 μM was the strongest. The extract showed moderate antioxidant capacity in DPPH assay method (IC50 = 100.2 μg/mL) and total phenolics and total flavonoids contents of the dried extract were also determined as 39.6 and 90.7 mg/g, respectively.