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      Comparative analysis using a mouse model of the immunogenicity of artificial VLP and attenuated Salmonella strain carrying a DNA-vaccine encoding HIV-1 polyepitope CTL-immunogen.

      Vaccine
      AIDS Vaccines, administration & dosage, immunology, Administration, Rectal, Animals, Cell Division, physiology, Culture Media, DNA, Viral, Enzyme-Linked Immunosorbent Assay, Epitopes, HIV-1, Injections, Intramuscular, Mice, Microscopy, Atomic Force, Plasmids, Salmonella, T-Lymphocytes, Cytotoxic, Vaccines, DNA

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          Abstract

          Two systems have been examined for delivery of DNA-vaccine encoding a HIV-1 polyepitope CTL-immunogen (TCI). One is intended for i.m. injection and is in the form of an artificial virus like particle containing eukaryotic expression plasmid pcDNA-TCI encapsulated within a spermidine-polyglucin conjugate. The other is intended for mucosal immunization and is based on attenuated Salmonella typhimurium strain 7207, which can deliver pcDNA-TCI directly into professional antigen-presenting cells (APC). After immunization, the artificial VLP and recombinant Salmonella induced an enhanced HIV specific serum antibody, proliferative and CTL responses compared to those induced by naked pcDNA-TCI. The most significant responses were produced when pcDNA-TCI was delivered by Salmonella.

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