CellProfiler: image analysis software for identifying and quantifying cell phenotypes

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

CellProfiler, the first free, open-source system for flexible and high-throughput cell image analysis is described.

Abstract

Biologists can now prepare and image thousands of samples per day using automation, enabling chemical screens and functional genomics (for example, using RNA interference). Here we describe the first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler. CellProfiler can address a variety of biological questions quantitatively, including standard assays (for example, cell count, size, per-cell protein levels) and complex morphological assays (for example, cell/organelle shape or subcellular patterns of DNA or protein staining).

Related collections

Most cited references 59

Record: found
Abstract: found
Article: not found

Causal protein-signaling networks derived from multiparameter single-cell data.

Karen Sachs, Omar Perez, Dana Pe'er … (2005)

Machine learning was applied for the automated derivation of causal influences in cellular signaling networks. This derivation relied on the simultaneous measurement of multiple phosphorylated protein and phospholipid components in thousands of individual primary human immune system cells. Perturbing these cells with molecular interventions drove the ordering of connections between pathway components, wherein Bayesian network computational methods automatically elucidated most of the traditionally reported signaling relationships and predicted novel interpathway network causalities, which we verified experimentally. Reconstruction of network models from physiologically relevant primary single cells might be applied to understanding native-state tissue signaling biology, complex drug actions, and dysfunctional signaling in diseased cells.

0 comments Cited 337 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Multidimensional drug profiling by automated microscopy.

Zachary Perlman, Michael Slack, Yan Feng … (2004)

We present a method for high-throughput cytological profiling by microscopy. Our system provides quantitative multidimensional measures of individual cell states over wide ranges of perturbations. We profile dose-dependent phenotypic effects of drugs in human cell culture with a titration-invariant similarity score (TISS). This method successfully categorized blinded drugs and suggested targets for drugs of uncertain mechanism. Multivariate single-cell analysis is a starting point for identifying relationships among drug effects at a systems level and a step toward phenotypic profiling at the single-cell level. Our methods will be useful for discovering the mechanism and predicting the toxicity of new drugs.

0 comments Cited 129 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

High-dimensional and large-scale phenotyping of yeast mutants.

Markus Aebi, Masashi Yukawa, Taro Saito … (2005)

One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.

0 comments Cited 103 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): Genome Biol

Title: Genome Biology

Publisher: BioMed Central (London )

ISSN (Print): 1465-6906

ISSN (Electronic): 1465-6914

Publication date (Print): 2006

Publication date (Electronic): 31 October 2006

Volume: 7

Issue: 10

Page: R100

Affiliations

[1 ]Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA

[2 ]Computer Sciences and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02142, USA

[3 ]Department of Radiology, Brigham and Women's Hospital, Boston, MA 02115, USA

[4 ]Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA

Article

Publisher ID: gb-2006-7-10-r100

DOI: 10.1186/gb-2006-7-10-r100

PMC ID: 1794559

PubMed ID: 17076895

SO-VID: 48b108a4-cdfc-4442-810c-d4a0d35ab68f

License:

This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

CellProfiler: image analysis software for identifying and quantifying cell phenotypes

Read this article at

Abstract

Abstract

Related collections

Evolutionary Cell Biology

Most cited references 59

Causal protein-signaling networks derived from multiparameter single-cell data.

Multidimensional drug profiling by automated microscopy.

High-dimensional and large-scale phenotyping of yeast mutants.

Author and article information

Journal

Affiliations

Article

Categories

Comments

Comment on this article

Similar content 39

Cited by 1,274

Most referenced authors 460