10
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      The Disappearance, Excretion, and Metabolism of Tritiated Prolyl-Leucyl-Glycinamide in Man

      research-article

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          <sup>3</sup>H-Pro-Leu-Gly-NH<sub>2</sub>, a melanocyte-stimulating hormone release inhibiting factor, was injected into 5 healthy men. A plot of the time of the disappearance of radioactivity in plasma wascharacteristic of a multiexponential curve. The half-life of the first component was 1.9 ± 0.06 min and of the second component 15.2 ± 1.5 min. The distribution volume was 20 % of the body weight. One hour after the injection of labeled MIF, 17.2 ± 0.7% of the dose of radioactivity was found in the urine and after 24 h, 25% had been excreted. Extraction of the urine, followed by chromatography on carboxymethyl cellulose and thin layer electrophoresis, showed that over 70% of the radioactivity was associated with the intact molecule while a smaller amount appeared to be either free proline, Pro-Leu-OH, or the deaminated form of the tripeptide. It appears that the main pathway for the clearance of MIF from the human body is excretion of the intact molecule.

          Related collections

          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1974
          1974
          20 March 2008
          : 16
          : 2
          : 119-126
          Affiliations
          Endocrine and Polypeptide Laboratories and Endocrinology Section of the Medical Service, Veterans Administration Hospital, and Department of Medicine, Tulane University School of Medicine, New Orleans, La., and Hospital General del Centro Medico Nacional, IMSS, Mexico, D.F.
          Article
          122558 Neuroendocrinology 1974;16:119–126
          10.1159/000122558
          4610443
          48e3b3af-6c66-4c0e-a292-c9b293e86df9
          © 1974 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 14 February 1974
          : 14 May 1974
          Page count
          Pages: 8
          Categories
          Paper

          Endocrinology & Diabetes,Neurology,Nutrition & Dietetics,Sexual medicine,Internal medicine,Pharmacology & Pharmaceutical medicine
          MIF,Pro-Leu-Gly-NH2

          Comments

          Comment on this article