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Burkholderia thailandensis: genetic manipulation
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Abstract
Burkholderia thailandensis is a Gram-negative bacterium endemic to southeast Asia and northern Australia soils. Non-pathogenic, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis.