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      Highly sensitive fluorescent pH microsensor based on the ratiometric dye pyranine immobilized on silica microparticles

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          Abstract

          Pyranine (HPTS) is a remarkably interesting pH sensitive dye that has been used for plenty of applications. Its high quantum yield and extremely sensitive ratiometric fluorescence against pH change makes it a very favorable for pH sensing applications and development of pH nano/microsensors. However, its strong negative charge and lack of easily modifiable functional groups makes it difficult to be used with charged substrates such as silica. This study reports a noncovalent HPTS immobilization methodology on silica microparticles that considers the retention of pH sensitivity as well as long term stability of the pH microsensors. The study emphasizes on importance of surface charge for governing the sensitivity of the immobilized HPTS dye molecules on silica microparticles. Importance of methodology of immobilization that preserves the sensitivity as well as stability of the microsensors is also assessed.

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          Author and article information

          Journal
          08 July 2021
          Article
          10.1002/chem.202101568
          2107.03672

          http://arxiv.org/licenses/nonexclusive-distrib/1.0/

          Custom metadata
          Chemistry - A European Journal, 2021
          8 pages, 4 figures, 1 table
          physics.chem-ph cond-mat.mtrl-sci

          Condensed matter, Physical chemistry

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