Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Subcellular distribution of mutant movement proteins of Cucumber mosaic virus fused to green fluorescent proteins.

The Journal of General Virology

metabolism, genetics, Viral Proteins, virology, Tobacco, Subcellular Fractions, Recombinant Fusion Proteins, Protoplasts, Plant Viral Movement Proteins, Plant Diseases, Mutation, Microscopy, Confocal, Green Fluorescent Proteins, pathogenicity, Cucumovirus

Read this article at

ScienceOpenPublisherPubMed
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      The subcellular distribution of the movement proteins (MPs) of nine alanine-scanning mutants of Cucumber mosaic virus (CMV), fused to the green fluorescent protein (GFP) and expressed from CMV, was determined by confocal microscopy of infected epidermal cells of Nicotiana tabacum and Nicotiana benthamiana, as well as infected N. benthamiana protoplasts. Only those mutant MPs that were functional for movement in all host species tested localized to plasmodesmata of infected epidermal cells and to tubules extending from the surface of infected protoplasts, as for wild-type CMV 3a MP. Various mutant MPs that were either conditionally functional for movement or dysfunctional for movement did not localize to plasmodesmata and did not form tubules on the surface of infected protoplasts. Rather, they showed distribution to different extents throughout the infected cells, including the cytoplasm, nucleus or the plasma membrane. The CMV 3a MP also did not associate with microtubules.

      Related collections

      Author and article information

      Journal
      10.1099/vir.0.80351-0
      15784916

      Comments

      Comment on this article