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Abstract
Using in situ hybridization, we have investigated the presence of mRNA coding for
growth hormone (GH) in the rat brain. Using 32P-labeled GH cDNA as a probe, the pituitary
gland showed hybridization in unfixed sections. Using 3H-labeled GH cDNA hybridized
to fixed sections, only cells in the anterior pituitary were labeled in good agreement
with the localization of somatotropes. In the brain, wide zones were labeled with
32P-GH cDNA: the caudate putamen, the striatum, the ventral thalamus, the formatio
reticularis and the basal cortex. With the 3H GH-cDNA probe, more discrete regions
of the brain showed hybridization: the basal cortex, the outside part of the hippocampus
and the caudate putamen.