18
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      The integrase cofactor LEDGF/p75 associates with Iws1 and Spt6 for postintegration silencing of HIV-1 gene expression in latently infected cells.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The persistence of a latent reservoir containing transcriptionally silent, but replication-competent, integrated provirus is a serious challenge to HIV eradication. HIV integration is under the control of LEDGF/p75, the cellular cofactor of viral integrase. Investigating possible postintegration roles for LEDGF/p75, we find that LEDGF/p75 represses HIV expression in latently infected cells. LEDGF/p75 associated with two proteins involved in the control of gene expression and chromatin structure, Spt6 and Iws1, to form a stable complex. Iws1 plays a role in the establishment of latent infection, whereas Spt6 functions to recruit Iws1 and LEDGF/p75 to the silenced provirus and maintains histone occupancy at the HIV promoter. In latently infected cells, depletion of the complex results in reactivation of HIV expression Altogether, our results indicate that a complex containing LEDGF/p75, Iws1, and Spt6 participates in regulating postintegration steps of HIV latency.

          Related collections

          Author and article information

          Journal
          Cell Host Microbe
          Cell host & microbe
          1934-6069
          1931-3128
          Jan 14 2015
          : 17
          : 1
          Affiliations
          [1 ] Inserm, U1016, Institut Cochin, 75014 Paris, France; CNRS, UMR8104, 75014 Paris, France; Université Paris Descartes, 75014 Paris, France. Electronic address: anabel.gerard@gmail.com.
          [2 ] Inserm, U1016, Institut Cochin, 75014 Paris, France; CNRS, UMR8104, 75014 Paris, France; Université Paris Descartes, 75014 Paris, France.
          [3 ] Hybrigenics, SA, Paris, 75014 France.
          [4 ] Inserm, U1016, Institut Cochin, 75014 Paris, France; CNRS, UMR8104, 75014 Paris, France; Université Paris Descartes, 75014 Paris, France. Electronic address: stephane.emiliani@inserm.fr.
          Article
          S1931-3128(14)00456-9
          10.1016/j.chom.2014.12.002
          25590759
          49c4ab99-158a-4f4d-8b9b-ff81efabb556
          Copyright © 2015 Elsevier Inc. All rights reserved.
          History

          Comments

          Comment on this article