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      Estrogen Regulates the Development of Brain-Derived Neurotrophic Factor mRNA and Protein in the Rat Hippocampus

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      The Journal of Neuroscience
      Society for Neuroscience

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          Abstract

          <p class="first" id="d2144991e128">During development, estrogen has a variety of effects on morphological and electrophysiological properties of hippocampal neurons. Brain-derived neurotrophic factor (BDNF) also plays an important role in the survival and differentiation of neurons during development. We examined the effects of gonadectomy with and without estrogen replacement on the mRNA and protein of BDNF and its receptor, trkB, during early postnatal development of the rat hippocampus. We used immunocytochemistry to demonstrate that estrogen receptor α (ERα) and BDNF were localized to the same cells within the developing hippocampus. BDNF and ERα were colocalized in pyramidal cells of the CA3 subregion and to a lesser extent in CA1. To determine whether BDNF mRNA was regulated by estrogen during development, we gonadectomized male rat pups at postnatal day 0 (P0) and examined mRNA and protein levels from P0 to P25 using real-time reverse transcription-PCR and Western blot analysis. After gonadectomy, BDNF mRNA levels are significantly reduced on P7, but after treatment of gonadectomized animals with estradiol benzoate on P0, levels at all ages were similar to those in intact animals. BDNF mRNA changes after gonadectomy are accompanied by an increase in the levels of BDNF protein, which were reduced by estrogen treatment at P0. We also examined the effect of postnatal estrogen treatment on trkB. There were no significant changes in trkB mRNA or protein in gonadectomized or estrogen-replaced animals. These results suggest that a direct interaction may exist between ERα and BDNF to alter hippocampal physiology during development in the rat. </p>

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          Author and article information

          Journal
          The Journal of Neuroscience
          J. Neurosci.
          Society for Neuroscience
          0270-6474
          1529-2401
          April 01 2002
          April 01 2002
          April 01 2002
          April 01 2002
          : 22
          : 7
          : 2650-2659
          Article
          10.1523/JNEUROSCI.22-07-02650.2002
          6758321
          11923430
          4a571466-72a6-402f-965b-72dc97c46061
          © 2002
          History

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