We present the first record of Plantago heterophylla Nutt. (Plantaginaceae) to Brazil, based on specimens from Rio Grande, Rio Grande do Sul, Brazil. The species is easily recognized due to its linear leaves, 2 stamens, and pixidium with 10–30 seeds. Plantago heterophylla is considered native from United States and Mexico, but it is distributed as adventitious in the east of Argentina, Paraguay, and Uruguay. Images from the species and a distribution map illustrating its occurrence in the American continent are provided.