23
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      The elucidation of novel capsular genotypes of Haemophilus influenzae type b with the polymerase chain reaction.

      1 , ,
      Journal of medical microbiology
      Microbiology Society

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Molecular characterization is an important pre-requisite for post-vaccine studies of Haemophilus influenzae type b (Hib). Three capsular genotyping patterns, b(S), b(G) and b(V), have been described in the major phylogenetic lineage of Hib. However, in a recent series of prospective studies, three new hybridisation patterns were observed among 425 strains of Hib. Four pairs of polymerase chain reaction (PCR) primers were used to identify the capsular gene (cap) structure of these Hib strains. This showed that the strains possessed simple DNA re-arrangements. In two instances a change in restriction enzyme recognition site was the most likely cause of the new hybridisation pattern. The third strain possessed a cap b locus consisting of intact tandem repeats of cap b in a b(S) background. It was reasoned that a similar cap b locus would not be readily recognised by hybridisation in a b(G) background, and b(G) strains were therefore characterized by the PCR method. This showed one of 35 b(G) strains to possess a cap locus with intact tandem repeat copies of cap b. The novel capsular genotypes described here are rare, but can be detected rapidly and accurately by a combination of PCR and capsular genotyping hybridisation patterns.

          Related collections

          Author and article information

          Journal
          J. Med. Microbiol.
          Journal of medical microbiology
          Microbiology Society
          0022-2615
          0022-2615
          Aug 1995
          : 43
          : 2
          Affiliations
          [1 ] Oxford Public Health Laboratory, John Radcliffe Hospital, Headington.
          Article
          10.1099/00222615-43-2-120
          7629851
          4a71adbf-8bb5-46f4-9cb1-3f2121603b19
          History

          Comments

          Comment on this article